Fields K L, Luria S E
J Bacteriol. 1969 Jan;97(1):64-77. doi: 10.1128/jb.97.1.64-77.1969.
Colicins E1 and K inhibited a whole series of energy-dependent reactions in Escherichia coli cells, including motility, biosynthesis of nucleic acids, proteins and polysaccharides, and the conversion of ornithine to citrulline. Respiration was only partially affected, and substrates such as glucose continued to be catabolized through the normal pathways, albeit with reduced CO(2) production. The soluble products of aerobic glucose catabolism by colicin-treated cells were analyzed. Pyruvate replaced acetate as the major excreted product, and the following intermediates of glycolysis were excreted in significant amounts: glucose-6-phosphate, fructose-1,6-diphosphate, dihydroxyacetone phosphate, and 3-phosphoglycerate. Anaerobically growing cells manifested a somewhat enhanced tolerance to the colicins. This protection by anaerobiosis appeared to depend on the exclusion of oxygen more than on the extent of fermentative catabolism versus catabolism of the respiratory type. These results are interpreted in terms of possible functions of colicin in lowering the adenosine triphosphate (ATP) content of the cells and in terms of the role of lowered ATP levels in inhibiting many of the energy-requiring reactions.
大肠杆菌素E1和K抑制大肠杆菌细胞中的一系列能量依赖性反应，包括运动性、核酸、蛋白质和多糖的生物合成，以及鸟氨酸向瓜氨酸的转化。呼吸作用仅受到部分影响，葡萄糖等底物继续通过正常途径进行分解代谢，尽管二氧化碳产生量减少。对经大肠杆菌素处理的细胞进行需氧葡萄糖分解代谢的可溶性产物分析。丙酮酸取代乙酸成为主要排泄产物，并且糖酵解的以下中间产物大量排泄：6-磷酸葡萄糖、1,6-二磷酸果糖、磷酸二羟丙酮和3-磷酸甘油酸。厌氧生长的细胞对大肠杆菌素表现出一定程度的耐受性增强。这种厌氧保护似乎更多地取决于氧气的排除，而不是发酵性分解代谢与呼吸型分解代谢的程度。这些结果是根据大肠杆菌素在降低细胞三磷酸腺苷（ATP）含量方面的可能功能以及降低的ATP水平在抑制许多能量需求反应中的作用来解释的。
