Otto T J, Trenkner M, Stopczyk A, Gawdziński M, Chelstowska B
Thorax. 1968 Nov;23(6):645-51. doi: 10.1136/thx.23.6.645.
In order to evaluate methods of preserving lungs for use in transplantation, experiments on 28 mongrel dogs were carried out. Two methods were tried—first, mechanical respiration of isolated lungs under deep hypothermia, with the vascular bed filled with blood; and, secondly, the perfusion of isolated lungs with the aid of a modified DeWall's apparatus. Allogenic transplantations of lungs preserved in both ways were carried out. Gasometric and histological examinations of preserved lungs, before and after transplantation, were performed. The best results were obtained with perfusion under hypothermic conditions; ventilation without perfusion resulted in failure. Lung transplantation was successful when, after being preserved, the lung remained unchanged. Major discrepancies between the macroscopic and microscopic findings in preserved lungs were observed. An original classification of the changes occurring in preserved lungs is proposed.
为了评估用于移植的肺保存方法，对28只杂种狗进行了实验。尝试了两种方法：第一，在深度低温下对离体肺进行机械通气，血管床充满血液；第二，借助改良的德瓦尔装置对离体肺进行灌注。对以这两种方式保存的肺进行了同种异体移植。对保存的肺在移植前后进行了气体分析和组织学检查。低温灌注取得了最佳效果；无灌注通气则导致失败。当肺在保存后保持不变时，肺移植成功。观察到保存的肺在宏观和微观发现之间存在重大差异。提出了保存的肺中发生变化的原始分类。
