Yokoo H, Craig R M, Harwood T R, Cochrane C
Gastroenterology. 1979 Nov;77(5):1082-7.
Griseofulvin was fed to male Swiss albino mice, which were sacrificed at varying times after the initiation of the feeding. The following were compared with mice fed a control diet: hepatic histology, hepatic weight, plasma glycocholate, glycolithocholate, cholesterol, bilirubin, and alkaline phosphatase. Concurrent with the development of hepatic protoporphyria, a progressive cholestatic lesion was produced with marked bile canalicular dilatation and elevation of the plasma bile salts, alkaline phosphatase, and cholesterol without a rise in bilirubin. Adaptation to the cholestatic injury occurred in about 60 days despite continued griseofulvin feeding. This was evidenced by decreased values in the biochemical profile with concomitant improvement in the bile canalicular morphology. Following this event of adaptation, Mallory bodies began to appear in the livers, often in the periphery of the hepatic lobule. This model may be useful in studying mechanisms of cholestasis, Mallory body formation, and their relationship to altered microtubular systems in the hepatocyte.
将灰黄霉素喂给雄性瑞士白化小鼠，在开始喂食后的不同时间将其处死。将以下各项与喂食对照饮食的小鼠进行比较：肝脏组织学、肝脏重量、血浆甘氨胆酸盐、甘氨石胆酸盐、胆固醇、胆红素和碱性磷酸酶。随着肝脏原卟啉症的发展，出现了进行性胆汁淤积性病变，伴有明显的胆小管扩张以及血浆胆汁盐、碱性磷酸酶和胆固醇升高，而胆红素未升高。尽管持续喂食灰黄霉素，但在约60天时出现了对胆汁淤积性损伤的适应。这表现为生化指标值降低，同时胆小管形态有所改善。在这种适应事件之后，马洛小体开始出现在肝脏中，通常位于肝小叶周边。该模型可能有助于研究胆汁淤积的机制、马洛小体的形成及其与肝细胞中微管系统改变的关系。
