Bissett M L, Powers C, Wood R M
Appl Microbiol. 1969 Apr;17(4):507-11. doi: 10.1128/am.17.4.507-511.1969.
Immunofluorescence and conventional bacteriological methods were compared for their ability to detect Salmonella typhi in 134 fecal specimens from 105 individuals associated with an outbreak of typhoid fever. Smears prepared from untreated fecal material (direct method) and after a preliminary incubation in selenite F broth (delayed method) were tested with an anti-Vi serum conjugated with fluorescein isothiocyanate. The delayed method was more sensitive than the direct method in detecting S. typhi. The delayed method was positive in 40 of 41 patients positive by culture methods, but gave positive or questionable reactions in 11 presumably uninfected individuals. The fluorescent-antibody test employing a Vi conjugate is a satisfactory screening procedure for detecting S. typhi, but all positives must be confirmed bacteriologically.
对105名与伤寒热暴发相关的个体的134份粪便标本，比较了免疫荧光法和传统细菌学方法检测伤寒沙门氏菌的能力。用异硫氰酸荧光素偶联的抗Vi血清检测未处理粪便材料制备的涂片（直接法）以及在亚硒酸盐F肉汤中初步培养后的涂片（延迟法）。延迟法在检测伤寒沙门氏菌方面比直接法更敏感。在41名培养方法呈阳性的患者中，延迟法有40例呈阳性，但在11名推测未感染的个体中出现了阳性或可疑反应。采用Vi偶联物的荧光抗体试验是检测伤寒沙门氏菌的一种令人满意的筛查程序，但所有阳性结果都必须通过细菌学方法进行确认。
