Thomason B M, McWhorter A C
Bull World Health Organ. 1965;33(5):681-5.
Fluorescent antibody techniques have proved advantageous for rapid detection of enteropathogenic Escherichia coli. Previous attempts to adapt these procedures to the rapid identification of Salmonella typhi have been limited by the number of cross-reactions obtained when a polyvalent Salmonella or a S. typhi O conjugate was used to stain faecal smears. The results obtained using a purified Vi conjugate and a S. typhi O, Vi conjugate to screen faecal smears from a number of typhoid carriers are described in this report. The data show that essentially the same number of carriers were found positive by fluorescent antibody as were found by conventional bacteriological methods, although each technique missed a number of positive specimens that were detected by the other. The immunofluorescent technique has the advantage of being more rapid and economical to perform than cultural procedures.
荧光抗体技术已被证明在快速检测肠道致病性大肠杆菌方面具有优势。先前尝试将这些方法应用于伤寒沙门氏菌的快速鉴定时，由于使用多价沙门氏菌或伤寒沙门氏菌O结合物对粪便涂片进行染色时出现的交叉反应数量而受到限制。本报告描述了使用纯化的Vi结合物和伤寒沙门氏菌O、Vi结合物对多名伤寒带菌者的粪便涂片进行筛查所获得的结果。数据表明，荧光抗体检测出的带菌者阳性数量与传统细菌学方法基本相同，尽管每种技术都遗漏了一些被另一种技术检测到的阳性标本。免疫荧光技术具有比培养程序更快速、更经济的优点。
