通过玻片凝集试验检测伤寒热患者尿液中的伤寒杆菌D、Vi和d抗原。
Detection of Salmonella typhi D, Vi, and d antigens, by slide coagglutination, in urine from patients with typhoid fever.
作者信息
Rockhill R C, Rumans L W, Lesmana M, Dennis D T
出版信息
J Clin Microbiol. 1980 Mar;11(3):213-6. doi: 10.1128/jcm.11.3.213-216.1980.
Abstract
Salmonella typhi antigens D, Vi, and d were detected in the urine of 59 out of 61 (97%) bacteriologically confirmed typhoid fever patients by slide coagglutination with monovalent antisera coupled to protein A-rich staphylococci. These antigens were also detected in the urine of an additional 22 patients, 16 of whom subsequently demonstrated seroconversion by S. typhi O antibody agglutination, but from whom the bacterium was not isolated. The remaining 13 patients had negative urine coagglutination results, no isolation of S. typhi from blood or stool specimens, and no demonstration of seroconversion. These results suggest that the method of slide coagglutination of urine can be used to screen patients with suspected typhoid fever with a high degree of reliability. The method may also have potential importance in the diagnosis of typhoid when the bacterium is not isolated.
摘要
采用与富含蛋白A的葡萄球菌偶联的单价抗血清进行玻片凝集试验，在61例细菌学确诊的伤寒热患者中，有59例（97%）的尿液中检测到伤寒杆菌抗原D、Vi和d。另外22例患者的尿液中也检测到了这些抗原，其中16例随后通过伤寒杆菌O抗体凝集试验显示血清学转化，但未分离出该细菌。其余13例患者尿液凝集试验结果为阴性，血液或粪便标本中未分离出伤寒杆菌，也未显示血清学转化。这些结果表明，尿液玻片凝集法可用于高度可靠地筛查疑似伤寒热患者。当未分离出细菌时，该方法在伤寒诊断中可能也具有潜在重要性。
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