Gokani V N, Thakker M U, Patel J G, Ghosh S K, Chatterjee S K
Indian J Physiol Pharmacol. 1979 Apr-Jun;23(2):101-4.
The present method describes the estimation of gamma-aminobutyric acid (GABA) from adult rat brain by thin layer chromatography (TLC). The major advantage of this TLC method is the sensitivity and repidity of estimation. About 93 to 98 percent recovery is possible by this procedure and this shows linearity up to 9 micrograms. Replicate analysis of GABA by this method shows a co-efficient variation of 1.31%
本方法描述了通过薄层色谱法(TLC)对成年大鼠脑中γ-氨基丁酸(GABA)的测定。该TLC方法的主要优点是测定的灵敏度和快速性。通过此程序可实现约93%至98%的回收率,并且在高达9微克时呈线性。用该方法对GABA进行重复分析显示变异系数为1.31%