脑中γ-氨基丁酸的灵敏且特异的测定
A sensitive and specific determination of gamma aminobutric acid in brain.
作者信息
Koćmierska-Grodzka D, Mazanowska A, Gerber G B
出版信息
Pol J Pharmacol Pharm. 1978 Jan-Feb;30(1):17-20.
PMID:643737
Abstract
A sensitive and specific method for the determination of gamma aminobutyric acid in small (30 mg) brain samples is described. It is based on preparation of the dansyl-butyrolactam (DBL), subsequent separation by thin layer chromatography and measurement of fluorescence after elution. Recovery is checked by routinely adding 14C labelled GABA.
摘要
本文描述了一种用于测定小体积（30毫克）脑样本中γ-氨基丁酸的灵敏且特异的方法。该方法基于丹磺酰丁内酰胺（DBL）的制备，随后通过薄层色谱法进行分离，并在洗脱后测量荧光。通过常规添加14C标记的GABA来检查回收率。
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