一种使用51Cr的微量细胞毒性试验，用于测量特异性细胞介导和体液免疫反应。

A microcytotoxicity assay using 51Cr for measuring specific cell-mediated and humoral immune responses.

作者信息

Della Penta D, Cipuzak P M, Phillips M E

出版信息

Int Arch Allergy Appl Immunol. 1979;60(3):332-45. doi: 10.1159/000232359.

Abstract

A microcytotoxicity assay (MCA) for measuring in vitro cell-mediated cytotoxicity (CMC) and humoral cytotoxicity which uses 51Cr-labelled target cells is described. Data is presented to show the uptake and rentention of 51Cr by various viable cultured cells. The CMC determined by 51Cr-labelled target cell retention in microtest II plate wells was verified by microscopic observation. Comparisons between the CMC observed 24 and 48 h after incubation of immune and normal lymphoid cells with 51Cr-labelled target cells were made. The factors, including savings in time and expense, which may make this modified MCA superior to other MCAs in use, are discussed.

摘要

描述了一种用于测量体外细胞介导的细胞毒性（CMC）和体液细胞毒性的微量细胞毒性测定法（MCA），该方法使用51Cr标记的靶细胞。给出的数据显示了各种活培养细胞对51Cr的摄取和保留情况。通过显微镜观察验证了在微量测试II板孔中51Cr标记的靶细胞保留所测定的CMC。对免疫淋巴细胞和正常淋巴细胞与51Cr标记的靶细胞孵育24小时和48小时后观察到的CMC进行了比较。讨论了可能使这种改良的MCA优于其他正在使用的MCA的因素，包括节省时间和费用。