Burton R, Thompson J, Warner N L
J Immunol Methods. 1975;8(1-2):133-49. doi: 10.1016/0022-1759(75)90090-3.
A microculture system for in vitro induction of tumour-specific immunity with syngeneic spleen cells and plasma-cell tumours is described. The system generates cytoxic lymphocytes assessed by their ability to lyse 51Cr-labelled target cells in a microcytotoxicity assay. Both the inductive and effector phases are subject to many variables in material and methodology and the optimal conditions are defined in this study. Of particular importance are the responder- to stimulator-cell ratios, the presence of 2-mercaptoethanol, the day of harvest, the method of target-cell 51Cr labelling, and preincubation of the cytotoxic lymphocytes prior to assay.
本文描述了一种用于体外诱导同基因脾细胞和浆细胞瘤肿瘤特异性免疫的微培养系统。该系统所产生的细胞毒性淋巴细胞通过在微细胞毒性试验中裂解51Cr标记靶细胞的能力来评估。诱导期和效应期在材料和方法上都存在许多变量，本研究确定了最佳条件。特别重要的是应答细胞与刺激细胞的比例、2-巯基乙醇的存在、收获日期、靶细胞51Cr标记方法以及细胞毒性淋巴细胞在测定前的预孵育。
