Boisvieux J F, Steimer J L, Venot A, Benhamou J P, Peignoux M, Lebrec D
Int J Biomed Comput. 1979 Aug;10(4):331-40. doi: 10.1016/0020-7101(79)90005-9.
In order to perform a quantification of the hepatic RES function, a mathematical model of the colloids phagocytosis was constructed and validated in normal and partially hepatectomised rats. The experimental design consisted of the collection of successive blood samples for the measurement of radiocolloids time courses after the injection of different doses of gelatin colloids (0.075--5 mg of gelatin/100 g body wt). The unknown parameters were estimated by method of maximum likelihood using a second order algorithm. A good fit between experimental and simulated data was obtained for a large range of injected doses using a single set of parameters. Comparisons of parameters values between normal and hepatectomised rats were found to be -onsistent with the hepatectomy ratio. This computerised estimation of parameters provides a determination of both the total number of Kupffer cells and the mean time of a complete phagocytosis cycle which cannot be obtained by classical approaches.
为了对肝脏的网状内皮系统（RES）功能进行量化，构建了胶体吞噬的数学模型，并在正常大鼠和部分肝切除大鼠中进行了验证。实验设计包括在注射不同剂量的明胶胶体（0.075 - 5mg明胶/100g体重）后，连续采集血样以测量放射性胶体的时间进程。使用二阶算法通过最大似然法估计未知参数。使用一组参数在大范围的注射剂量下，实验数据和模拟数据之间取得了良好的拟合。发现正常大鼠和肝切除大鼠之间参数值的比较与肝切除比例一致。这种参数的计算机化估计提供了对库普弗细胞总数和完整吞噬循环平均时间的测定，而这是传统方法无法获得的。
