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钩端螺旋体轴丝的纯化、超微结构及组成

Purification, ultrastructure, and composition of axial filaments from Leptospira.

作者信息

Nauman R K, Holt S C, Cox C D

出版信息

J Bacteriol. 1969 Apr;98(1):264-80. doi: 10.1128/jb.98.1.264-280.1969.

Abstract

The ultrastructure of three strains of water Leptospira was studied by negative staining, thin sectioning, and freeze-etching. The cells possessed a triple-layered sheath which covered two independent axial filaments, one inserted subterminally in each end of the cell. The protoplasmic cylinder was surrounded by a triple-layered cell wall and possessed ribosomes, lamellar structures, and a typical procaryotic nuclear region. The axial filament was comprised of several component structures. An axial fibril, with a diameter of 20 to 25 nm, consisted of a solid inner core (13 to 16 nm in diameter) surrounded by a coat. A terminal knob (40 to 70 nm in length) was connected to a series of disc insertion structures at the terminal end of the axial fibril. The axial fibril was surrounded by a helical outer coat (35 to 60 nm in diameter) which was composed of a continuously coiled fiber, 3 to 4 nm in diameter, embedded in an electron-dense material. A procedure for the purification of the axial fibrils was presented and their ultrastructural, physical, and chemical properties were determined. Similarities in ultrastructural, physical, and chemical properties were noted between the axial fibrils and bacterial flagella. A schematic model of the leptospiral axial filament is presented, and a mechanism is proposed for its function as a locomotor organelle.

摘要

通过负染色、超薄切片和冷冻蚀刻技术对三株水生钩端螺旋体的超微结构进行了研究。细胞具有三层鞘膜,包裹着两根独立的轴丝,一根轴丝亚末端插入细胞的每一端。原生质圆柱体被三层细胞壁包围,含有核糖体、层状结构和典型的原核生物核区。轴丝由几种组成结构构成。一条直径为20至25纳米的轴纤丝,由一个实心的内核(直径13至16纳米)和一层包膜组成。一个末端球(长度为40至70纳米)与轴纤丝末端的一系列盘状插入结构相连。轴纤丝被一层螺旋状的外套(直径35至60纳米)包围,该外套由直径为3至4纳米的连续盘绕纤维嵌入电子致密物质中组成。文中介绍了轴纤丝的纯化方法,并测定了其超微结构、物理和化学性质。轴纤丝与细菌鞭毛在超微结构、物理和化学性质上存在相似之处。文中给出了钩端螺旋体轴丝的示意图模型,并提出了其作为运动细胞器发挥功能的机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/15d6/249933/57e621336427/jbacter00390-0298-a.jpg

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