Pai C H
J Bacteriol. 1969 Sep;99(3):696-701. doi: 10.1128/jb.99.3.696-701.1969.
Cell-free extracts of Escherichia coli were active in catalyzing the synthesis of a biotin vitamer from 7,8-diaminopelargonic acid. The vitamer was identified as desthiobiotin on the basis of its chromatographic and electrophoretic characteristics and its biotin activities for a variety of microorganisms. The reaction was stimulated five-fold by bicarbonate, suggesting that an "active CO(2)" was incorporated into the carbonyl carbon of desthiobiotin. The enzyme was demonstrable in a wild-type (K-12) and in all biotin mutants of E. coli that were tested, with the exception of a strain which was able to grow on desthiobiotin but not on diaminopelargonic acid. Furthermore, the enzyme was repressible by biotin in all of the strains tested. These results are consistent with the hypothesis that the biosynthesis of desthiobiotin from 7,8-diaminopelargonic acid is an obligatory step in the biosynthetic pathway of biotin in E. coli.
大肠杆菌的无细胞提取物能够催化由7,8 - 二氨基壬酸合成生物素维生素类似物。根据其色谱和电泳特性以及对多种微生物的生物素活性，该维生素类似物被鉴定为脱硫生物素。碳酸氢盐可使该反应速率提高五倍，这表明“活性CO₂”被掺入到脱硫生物素的羰基碳中。在野生型（K - 12）以及所有经过测试的大肠杆菌生物素突变体中都能检测到这种酶，但有一个菌株除外，该菌株能够在脱硫生物素上生长，却不能在二氨基壬酸上生长。此外，在所有测试菌株中，这种酶都可被生物素抑制。这些结果与以下假设一致，即从7,8 - 二氨基壬酸生物合成脱硫生物素是大肠杆菌生物素生物合成途径中的一个必要步骤。
