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大肠杆菌在镁饥饿期间质膜的变化。

Changes in the plasma membrane of Escherichia coli during magnesium starvation.

作者信息

Fiil A, Branton D

出版信息

J Bacteriol. 1969 Jun;98(3):1320-7. doi: 10.1128/jb.98.3.1320-1327.1969.

DOI:10.1128/jb.98.3.1320-1327.1969
PMID:4892379
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC315329/
Abstract

The effect of Mg(++) starvation on the structure of the Escherichia coli cell membrane was studied with the freeze-etch technique. Special attention was paid to changes within the plane of the membrane, which in normal exponentially growing cells has a netlike arrangement of particles 2 to 6 nm in diameter. During Mg(++) starvation, a paracrystalline particle pattern appeared on the plasma membrane, and large areas devoid of particles were seen. Although these changes are reproducibly associated with Mg(++) starvation of the bacteria, no decrease in the Mg(++) content of the cell envelope per se was detected, even after 24 hr of Mg(++) deprivation. The structural changes caused by Mg(++) deprivation appeared to involve specific and permanent alterations in membrane development. The absence of other nutrients or divalent cations did not induce similar alterations.

摘要

采用冷冻蚀刻技术研究了Mg(++)饥饿对大肠杆菌细胞膜结构的影响。特别关注了膜平面内的变化，在正常指数生长的细胞中，膜平面有直径为2至6纳米的颗粒呈网状排列。在Mg(++)饥饿期间，质膜上出现了准晶颗粒图案，并且可以看到大片无颗粒区域。尽管这些变化与细菌的Mg(++)饥饿可重复相关，但即使在Mg(++)剥夺24小时后，也未检测到细胞包膜本身的Mg(++)含量降低。Mg(++)剥夺引起的结构变化似乎涉及膜发育中的特定和永久性改变。缺乏其他营养物质或二价阳离子不会诱导类似的改变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0129/315329/5db3f8844782/jbacter00585-0508-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0129/315329/85171bd2cdbb/jbacter00585-0504-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0129/315329/12f63410b777/jbacter00585-0506-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0129/315329/611e3f081ec5/jbacter00585-0507-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0129/315329/5db3f8844782/jbacter00585-0508-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0129/315329/85171bd2cdbb/jbacter00585-0504-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0129/315329/12f63410b777/jbacter00585-0506-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0129/315329/611e3f081ec5/jbacter00585-0507-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0129/315329/5db3f8844782/jbacter00585-0508-a.jpg

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