大肠杆菌B-r中DNA合成的起源与方向
Origin and direction of DNA synthesis in E. coli B-r.
作者信息
Ward C B, Glaser D A
出版信息
Proc Natl Acad Sci U S A. 1969 Mar;62(3):881-6. doi: 10.1073/pnas.62.3.881.
Abstract
Replication of the chromosome of E. coli B/r has been studied by measuring the change with cell age of the rates of reversion to wild type of auxotrophic markers induced by nitrosoguanidine. Synchronous populations of bacteria were obtained by the procedure of Helmstetter and Cummings. The origin of DNA synthesis was determined to occur at 49 minutes +/- 5 minutes on the genetic map, and the direction of synthesis was found to be clockwise.
摘要
通过测量亚硝基胍诱导的营养缺陷型标记回复为野生型的速率随细胞年龄的变化，对大肠杆菌B/r染色体的复制进行了研究。通过Helmstetter和Cummings的方法获得了细菌同步群体。确定DNA合成起点在遗传图谱上49分钟±5分钟处，且合成方向为顺时针。
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