大肠杆菌核酸内切酶II。I. 分离与纯化。
Endonuclease II of E. coli. I. Isolation and purification.
作者信息
Friedberg E C, Goldthwait D A
出版信息
Proc Natl Acad Sci U S A. 1969 Mar;62(3):934-40. doi: 10.1073/pnas.62.3.934.
Abstract
The isolation and purification of a new endonuclease of E. coli is described. This enzyme degrades alkylated DNA as assayed by a technique that requires double-strand scission. The enzyme also makes a limited number of single-strand breaks in native nonalkylated DNA.
摘要
本文描述了从大肠杆菌中分离和纯化一种新的核酸内切酶的过程。通过一种需要双链断裂的技术检测发现，这种酶能降解烷基化的DNA。该酶还会在天然的非烷基化DNA中产生有限数量的单链断裂。
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