Gartner T K, Orias E, Lannan J E, Beeson J, Reid P J
Proc Natl Acad Sci U S A. 1969 Mar;62(3):946-51. doi: 10.1073/pnas.62.3.946.
Escherichia coli K12 2320(lambda)-15B has a mutation that results in ochre suppressor activity.(1) This mutation concomitantly causes a decreased growth rate in rich medium, an increased sensitivity to streptomycin,(1) and the production of some altered 30S ribosomes which are differentially sensitive to RNase.(2) The results presented below demonstrate that the molecules which cause suppression are tRNA. These observations justify the conclusions that the suppressor mutation did not occur in a structural gene for a ribosomal component, and that the decreased growth rate in rich medium, the increased sensitivity to streptomycin, and the production of altered 30S ribosomes are probably all secondary consequences of the suppressor mutation.
大肠杆菌K12 2320（λ）-15B发生了一种导致赭石抑制活性的突变。（1）这种突变同时导致在丰富培养基中生长速率降低、对链霉素敏感性增加，（1）并产生一些对核糖核酸酶敏感性不同的改变的30S核糖体。（2）下面给出的结果表明，导致抑制的分子是转运核糖核酸。这些观察结果证明了以下结论是合理的：抑制突变并非发生在核糖体组分的结构基因中，并且在丰富培养基中生长速率降低、对链霉素敏感性增加以及产生改变的30S核糖体可能都是抑制突变的次生后果。
