Gall J G, Pardue M L
Proc Natl Acad Sci U S A. 1969 Jun;63(2):378-83. doi: 10.1073/pnas.63.2.378.
A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-labeled RNA. The hybrids are detected by autoradiography. The technique is illustrated by the hybridization of ribosomal RNA to the amplified ribosomal genes in oocytes of the toad Xenopus. A low level of gene amplification was also detected in premeiotic nuclei (oogonia).
本文描述了一种在溶液中的RNA与完整细胞学制剂的DNA之间形成分子杂交体的技术。常规组织压片中的细胞被固定在一层薄琼脂下。接下来，用碱处理使DNA变性，然后与氚标记的RNA孵育。通过放射自显影检测杂交体。以蟾蜍非洲爪蟾卵母细胞中核糖体RNA与扩增的核糖体基因的杂交为例说明了该技术。在减数分裂前的细胞核（卵原细胞）中也检测到了低水平的基因扩增。
