Pellicciari Carlo
Eur J Histochem. 2017 Oct 19;61(4). doi: 10.4081/ejh.2017.2885. Epub 2017 Dec 22.
Especially in the latest years, histochemical investigations have progressively been oriented toward the visualization and quantitative assessment of single molecules, thanks to the availability of stains, reactions and procedures allowing to detect in situ proteins, or carboydrates or nucleic acid sequences with high specificity. This is evident from the recent literature, where in the large majority of the published articles immunohistochemistry, lectin histochemistry or fluorescence in situ hybridization were used as experimental methodologies. Since in biomedical research it is crucial to specifically label and localize molecules there, where they exert their structural roles and activities, histochemistry will continue to provide scientists the most appropriate tools for tracing molecular maps suitable for reaching a mechanistic explanation of cell functions in tissues.
尤其是近年来，由于有了能以高特异性原位检测蛋白质、碳水化合物或核酸序列的染色剂、反应和程序，组织化学研究逐渐朝着单个分子的可视化和定量评估方向发展。这在最近的文献中很明显，其中绝大多数已发表的文章都将免疫组织化学、凝集素组织化学或荧光原位杂交用作实验方法。由于在生物医学研究中，在分子发挥其结构作用和活性的地方对其进行特异性标记和定位至关重要，因此组织化学将继续为科学家提供最合适的工具，用于绘制分子图谱，以对组织中的细胞功能进行机制性解释。
