从大肠杆菌中分离腺苷酸环化酶。
Isolation of adenyl cyclase from Escherichia coli.
作者信息
Tao M, Lipmann F
出版信息
Proc Natl Acad Sci U S A. 1969 May;63(1):86-92. doi: 10.1073/pnas.63.1.86.
Abstract
We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg(++) requirement and is inhibited by fluoride and inorganic pyrophosphate.
摘要
我们发现了一种可溶性环化酶，使用放射性标记的ATP作为前体进行测定。反应产物通过薄层色谱法分离，并通过特异性降解进行鉴定。匀浆后，部分活性保留在颗粒部分，但很容易提取出来。通过常规方法将环化酶浓缩了100倍。该酶需要Mg(++)，并受到氟化物和无机焦磷酸的抑制。
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