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木瓜蛋白酶氨基酸序列的测定完成。

Completion of the amino acid sequence of papain.

作者信息

Husain S S, Lowe G

出版信息

Biochem J. 1969 Sep;114(2):279-88. doi: 10.1042/bj1140279.

DOI:10.1042/bj1140279
PMID:4898282
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1184853/
Abstract

Papain was inhibited with bromo[2-(14)C]acetic acid, the tertiary structure of the inhibited enzyme was unfolded and the disulphide bridges were reduced with mercaptoethanol and aminoethylated. Digestion with trypsin gave a radioactive peptide consisting of residues 18-58 inclusive and containing therefore the sequence of the thirteen unknown residues 29-41 in the primary sequence of papain. This peptide was digested with pepsin to give a radioactive peptide consisting of residues 18-47, which after digestion with 0.4m-hydrochloric acid gave a radioactive peptide consisting of residues 24-43 inclusive. Further digestion with 6m-hydrochloric acid gave peptides that were used to determine the sequence: Ser-Ala-Val-Val-Thr-Ile-Glx-Gly-Ile-Ile-Lys-Ile-Arg for the residues 29-41, so completing the amino acid sequence of papain.

摘要

用溴代[2-(14)C]乙酸抑制木瓜蛋白酶，使被抑制酶的三级结构展开，并用巯基乙醇还原二硫键并进行氨乙基化。用胰蛋白酶消化得到一个放射性肽段，其包含18至58位残基，因此包含木瓜蛋白酶一级序列中13个未知残基29至41的序列。该肽段用胃蛋白酶消化得到一个由18至47位残基组成的放射性肽段，用0.4m盐酸消化后得到一个由24至43位残基（含）组成的放射性肽段。用6m盐酸进一步消化得到用于确定序列的肽段：29至41位残基的序列为Ser-Ala-Val-Val-Thr-Ile-Glx-Gly-Ile-Ile-Lys-Ile-Arg，从而完成了木瓜蛋白酶的氨基酸序列测定。

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PAPAIN-CATALYSED HYDROLYSIS OF SOME HIPPURIC ESTERS. A NEW MECHANISM FOR PAPAIN-CATALYSED HYDROLYSIS.木瓜蛋白酶催化某些马尿酸酯的水解。木瓜蛋白酶催化水解的新机制。
Biochem J. 1965 Jul;96(1):199-204. doi: 10.1042/bj0960199.
2
CURRENT STATUS OF THE STRUCTURE OF PAPAIN: THE LINEAR SEQUENCE, ACTIVE SULFHYDRYL GROUP, AND THE DISULFIDE BRIDGES.木瓜蛋白酶结构的当前状态：线性序列、活性巯基和二硫键。
Proc Natl Acad Sci U S A. 1964 Nov;52(5):1276-83. doi: 10.1073/pnas.52.5.1276.
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ION-EXCHANGE CHROMATOGRAPHY OF AMINO-ACIDS: IMPROVEMENTS IN THE SINGLE COLUMN SYSTEM.氨基酸的离子交换色谱法：单柱系统的改进
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4
Phenolic hydroxyl ionization in papain.木瓜蛋白酶中的酚羟基离子化
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5
Peptides obtained by tryptic hydrolysis of performic acid-oxidized ribonuclease.通过对过甲酸氧化的核糖核酸酶进行胰蛋白酶水解得到的肽段。
J Biol Chem. 1956 Apr;219(2):623-42.
6
Crystalline papain. I. Preparation, specificity, and activation.结晶木瓜蛋白酶。I. 制备、特异性及激活
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On the aminoethylation of proteins.论蛋白质的氨乙基化
J Biol Chem. 1966 Aug 10;241(15):3457-61.
8
Structure of papain.木瓜蛋白酶的结构。
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