猪胰弹性蛋白酶的一级结构。N 端和二硫键。
The primary structure of porcine pancreatic elastase. The N-terminus and disulphide bridges.
作者信息
Brown J R, Kauffman D L, Hartley B S
出版信息
Biochem J. 1967 May;103(2):497-507. doi: 10.1042/bj1030497.
Abstract
- The amino acid composition and N-terminal groups of purified elastase show that it is a single peptide chain of 234 residues. 2. The N-terminal sequence is Val-Val-Gly-Gly-Thr-Glu-. 3. The sequences around the four disulphide bridges were determined by using a ;diagonal' electrophoretic technique. 4. These four bridges are homologous with the four common to bovine trypsin and chymotrypsin. 5. Out of 83 residues of the elastase sequence so far determined, 43 are homologous with similar regions of trypsin and chymotrypsin. 6. The evolutionary ancestry of these enzymes is discussed.
摘要
- 纯化弹性蛋白酶的氨基酸组成和N端基团表明它是一条由234个残基组成的单肽链。2. N端序列为Val-Val-Gly-Gly-Thr-Glu-。3. 利用“对角线”电泳技术确定了四条二硫键周围的序列。4. 这四条二硫键与牛胰蛋白酶和胰凝乳蛋白酶共有的四条二硫键同源。5. 在目前已确定的弹性蛋白酶序列的83个残基中，有43个与胰蛋白酶和胰凝乳蛋白酶的相似区域同源。6. 讨论了这些酶的进化起源。
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