大肠杆菌素CA42-E2的吸附动力学及其杀菌活性的逆转
Kinetics of adsorption of colicin CA42-E2 and reversal of its bactericidal activity.
作者信息
Reynolds B L, Reeves P R
出版信息
J Bacteriol. 1969 Oct;100(1):301-9. doi: 10.1128/jb.100.1.301-309.1969.
Abstract
The kinetics of killing of Escherichia coli K-12 by colicin CA42-E2 have been studied, and the data were used to estimate the adsorption constant of this colicin under various environmental conditions. Evidence was obtained suggesting that the adsorption of colicin occurred in two stages; the earlier stage was reversible and did not lead to the death of the cell, the latter stage was irreversible and bactericidal. Cells which had adsorbed a lethal quantity of colicin could be rescued for a short time by inactivating the adsorbed colicin with trypsin. However, when the metabolic activity of the cells was totally arrested the lethal effect of adsorbed colicin was subject to trypsin reversal over long periods of time.
摘要
已研究了大肠菌素CA42-E2对大肠杆菌K-12的杀伤动力学，并利用这些数据估算了该大肠菌素在各种环境条件下的吸附常数。获得的证据表明，大肠菌素的吸附分两个阶段进行；早期阶段是可逆的，不会导致细胞死亡，后期阶段是不可逆的且具有杀菌作用。吸附了致死量大肠菌素的细胞可以通过用胰蛋白酶使吸附的大肠菌素失活而在短时间内得到挽救。然而，当细胞的代谢活性完全停止时，吸附的大肠菌素的致死作用在很长一段时间内都可被胰蛋白酶逆转。
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