大肠杆菌对大肠杆菌素E2 - P9敏感菌株细胞的特异性吸附的可逆性。
Reversibility of the specific adsorption of colicin E2-P9 to cells of colicin-sensitive strains of Escherichia coli.
作者信息
Shannon R, Hedges A J
出版信息
J Bacteriol. 1973 Dec;116(3):1136-44. doi: 10.1128/jb.116.3.1136-1144.1973.
Abstract
The adsorption of colicin E2-P9 to its specific receptors on cells of sensitive strains of Escherichia coli is reversible under normal experimental conditions. At temperatures above 20 C, colicin may desorb from one cell and be readsorbed by a second with potentially lethal consequences. However, desorption of colicin seems unable to rescue a cell once it has received a lethal dose. These findings have implications both for the nature and types of specific receptors, and for the assay of colicin by the survivor count (lethal unit) methods.
摘要
在正常实验条件下，大肠杆菌素E2-P9与其在大肠杆菌敏感菌株细胞上的特异性受体的吸附是可逆的。在20℃以上的温度下，大肠杆菌素可能从一个细胞上解吸，并被另一个细胞重新吸附，这可能会产生致命后果。然而，一旦细胞接受了致死剂量，大肠杆菌素的解吸似乎无法挽救该细胞。这些发现对特异性受体的性质和类型以及通过存活计数（致死单位）方法测定大肠杆菌素都有影响。
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