Raney J P, McLennan B D
J Immunol Methods. 1979;29(1):65-70. doi: 10.1016/0022-1759(79)90126-1.
A modification of the standard quantitative precipitin test is described. The new procedure, which utilizes polyethylene glycol (PEG) at both the 37 degrees C and 4 degrees C incubation stages, provides a convenient quantitative precipitin test assay which is more reliable and much faster than the standard assay. Moreover, the modified or PEG assay gives a quantitative measure of antibody concentration even when antisera of low titre are tested. The sensitivity of the capillary-tube test is also enhanced when the supernatant solutions contain PEG.
本文描述了一种对标准定量沉淀试验的改进方法。新方法在37℃和4℃孵育阶段均使用聚乙二醇（PEG），提供了一种便捷的定量沉淀试验检测方法，该方法比标准检测方法更可靠且速度快得多。此外，即使检测低滴度抗血清，改进后的或PEG检测方法也能对抗体浓度进行定量测定。当上清液中含有PEG时，毛细管试验的灵敏度也会提高。
