用于定量检测抗I型、II型、III型胶原蛋白特异性抗体的酶联免疫吸附微测定法。
Enzyme-linked immunosorbent microassay for quantification of specific antibodies to collagen type I, II, III.
作者信息
Gosslau B, Barrach H J
出版信息
J Immunol Methods. 1979;29(1):71-7. doi: 10.1016/0022-1759(79)90127-3.
An enzyme-liked immunosorbent assay (ELISA) is described for determination of antibodies to collagen type I, II, III in serum or other body fluids. Polystyrene microplates are coated with either collagen I, II or III, and samples of body fluids being tested are incubated in the plates. Antibodies which attach specifically to the collagen are detected with anti-IgG antibody conjugated with peroxidase. Bound peroxidase is quantitatively estimated by the colour reaction produced with the substrate 5-aminosalicylic acid.
本文介绍了一种酶联免疫吸附测定（ELISA）法，用于测定血清或其他体液中针对I型、II型、III型胶原蛋白的抗体。将I型、II型或III型胶原蛋白包被在聚苯乙烯微孔板上，然后将待测体液样本在微孔板中孵育。用与过氧化物酶偶联的抗IgG抗体检测特异性结合胶原蛋白的抗体。通过底物5-氨基水杨酸产生的颜色反应对结合的过氧化物酶进行定量评估。
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