使用¹⁴C标记的胶原蛋白进行抗胶原蛋白抗体的放射免疫测定。

Radioimmunoassay for anticollagen--antibodies using 14C-labelled collagen.

作者信息

Menzel J

出版信息

J Immunol Methods. 1977;15(1):77-95. doi: 10.1016/0022-1759(77)90019-9.

Abstract

A sensitive radioimmunoassay for anticollagen antibodies is described. 14C-labelled human acid-soluble collagen of high specific activity (5 X 10(6) dpm/mg) is used as antigen either in native or denatured state. Experimentally induced anticollagen antibodies or RA synovial fluids containing antibodies to collagen are reacted with the labelled antigen. The immune complexes formed are precipitated with goat antiserum to rabbit globulins ('second antibody'). A systematic investigation of the labelled collagen in regard to cleavage by enzymes, fibril formation and specificity showed that no gross alteration had been caused by the labelling procedure. The assay furnishes information on the avidity, specificity and immunoglobulin class of experimental or pathological anticollagen antibodies. It can also be used as sensitive assay for collagen in biological fluids.

摘要

本文描述了一种用于抗胶原蛋白抗体的灵敏放射免疫测定法。具有高比活性（5×10⁶ dpm/mg）的¹⁴C标记的人酸溶性胶原蛋白，以天然或变性状态用作抗原。实验诱导的抗胶原蛋白抗体或含有抗胶原蛋白抗体的类风湿性关节炎滑膜液与标记抗原反应。形成的免疫复合物用山羊抗兔球蛋白抗血清（“第二抗体”）沉淀。对标记胶原蛋白在酶切、原纤维形成和特异性方面进行的系统研究表明，标记过程未引起明显改变。该测定法可提供有关实验性或病理性抗胶原蛋白抗体的亲和力、特异性和免疫球蛋白类别的信息。它还可用作生物体液中胶原蛋白的灵敏测定法。