Staines N A, Hardingham T, Smith M, Henderson B
Immunology. 1981 Dec;44(4):737-44.
Inbred Wistar strain rats developed a polyarthritic disease when injected intradermally with Freund's incomplete adjuvant (FIA) containing porcine or bovine type II collagen (CII). In contrast, neither allogeneic rat CII nor porcine proteoglycan monomer were arthritogenic, although both were, to some extent, immunogenic. Animals passively transfused with immune serum containing anti-CII antibodies did not develop arthritis and showed greatly reduced anti-CII humoral immune responses (measured by an enzyme-linked immunosorbent assay) when the serum was administered at the time of and subsequent to challenge with arthritogenic porcine CII. Similarly, the intravenous injection of animals sequentially with 100 microgram soluble CII and 1 ml immune anti-CII antiserum, 9 and 8 days respectively before challenge with arthritogenic CII, abrogated the arthritic response and depressed the humoral anti-porcine CII and anti-rat CII antibody titres more than tenfold. It is concluded that the immune status of the recipient rats with respect to CII is crucially important in determining the nature of the immune and arthritic response to CII appropriately administered in FIA.
近交系Wistar大鼠皮内注射含有猪或牛II型胶原（CII）的弗氏不完全佐剂（FIA）后会患上多关节炎疾病。相比之下，同种异体大鼠CII和猪蛋白聚糖单体都不会引发关节炎，尽管在某种程度上它们都具有免疫原性。用含有抗CII抗体的免疫血清进行被动输血的动物不会患上关节炎，并且在注射致关节炎性猪CII时及之后给予血清时，其抗CII体液免疫反应（通过酶联免疫吸附测定法测量）大幅降低。同样，在用致关节炎性CII攻击前9天和8天，分别给动物静脉注射100微克可溶性CII和1毫升免疫抗CII抗血清，可消除关节炎反应，并使体液抗猪CII和抗大鼠CII抗体滴度降低十倍以上。结论是，受体大鼠相对于CII的免疫状态对于确定在FIA中适当给予CII时免疫和关节炎反应的性质至关重要。
