转运RNA：对海拉细胞胞质、海拉细胞线粒体组分及大肠杆菌中的转运RNA进行凝胶电泳比较。
Transfer RNA: A comparison by gel electrophoresis of the tRNA in HeLa cytoplasm, HeLa mitochondrial fraction, and E. coli.
作者信息
Knight E, Sugiyama T
出版信息
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1383-8. doi: 10.1073/pnas.63.4.1383.
Abstract
A class of transfer RNA separable from the majority of transfer RNA by gel electrophoresis has been observed in E. coli and in the mitochondrial fraction of HeLa cells but not in HeLa cytoplasm. The size of this class of transfer RNA, as judged by its mobility on polyacrylamide gel, is intermediate between the major part of the transfer RNA and 5S RNA. It exists in part associated with the ribosomes, and its amino acid acceptor activity is different from the major class of transfer RNA.
摘要
在大肠杆菌以及海拉细胞的线粒体组分中观察到了一类可通过凝胶电泳与大多数转移核糖核酸分离的转移核糖核酸，但在海拉细胞的细胞质中未观察到。根据其在聚丙烯酰胺凝胶上的迁移率判断，这类转移核糖核酸的大小介于转移核糖核酸的主要部分和5S核糖核酸之间。它部分与核糖体结合存在，并且其氨基酸接受活性与主要类别的转移核糖核酸不同。
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Transfer RNA: A comparison by gel electrophoresis of the tRNA in HeLa cytoplasm, HeLa mitochondrial fraction, and E. coli.转运RNA：对海拉细胞胞质、海拉细胞线粒体组分及大肠杆菌中的转运RNA进行凝胶电泳比较。
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本文引用的文献
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Mitochondrial transfer ribonucleic acids.线粒体转移核糖核酸
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The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
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The cytoplasmic RNA of HeLa cells: new discrete species associated with mitochondria.海拉细胞的细胞质RNA：与线粒体相关的新离散种类。
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Dimers of alanine transfer RNA with acceptor activity.具有受体活性的丙氨酸转运RNA二聚体。
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10
Control of translation of MS2 RNA cistrons by MS2 coat protein.MS2 外壳蛋白对 MS2 RNA 顺反子翻译的调控
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