胞外多糖柯氏酸及其在肠杆菌科中的存在情况。
Exopolysaccharide colanic acid and its occurrence in the Enterobacteriaceae.
作者信息
Grant W D, Sutherland I W, Wilkinson J F
出版信息
J Bacteriol. 1969 Dec;100(3):1187-93. doi: 10.1128/jb.100.3.1187-1193.1969.
Abstract
A study of strains from the genera Salmonella, Escherichia, and Aerobacter has shown that under appropriate conditions many strains produce an exopolysaccharide slime of identical composition, which has been identified as colanic acid on the basis of its chemical composition and its sensitivity to certain bacteriophage-induced depolymerase enzymes. Chemical analysis shows that the polysaccharide contains O-acetyl groups in addition to the sugars glucose, galactose, fucose, and glucuronic acid. Mild acid hydrolysis has led to the isolation of a beta-glucosylfucose in addition to glucuronic acid containing oligosaccharides. Many strains were found to synthesize colanic acid under normal conditions of growth or under conditions favoring polysaccharide synthesis, whereas others only synthesized colanic acid when the control mechanism was derepressed by p-fluorophenylalanine.
摘要
一项对沙门氏菌属、大肠杆菌属和气杆菌属菌株的研究表明，在适当条件下，许多菌株会产生成分相同的胞外多糖黏液，根据其化学成分及其对某些噬菌体诱导的解聚酶的敏感性，该黏液已被鉴定为结肠酸。化学分析表明，除了葡萄糖、半乳糖、岩藻糖和葡萄糖醛酸外，该多糖还含有O - 乙酰基。温和的酸水解除了产生含葡萄糖醛酸的寡糖外，还分离出了一种β - 葡糖基岩藻糖。许多菌株在正常生长条件下或有利于多糖合成的条件下会合成结肠酸，而其他一些菌株只有在对氟苯丙氨酸解除控制机制的抑制时才会合成结肠酸。
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