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原噬菌体λ与溶原性细胞中细菌染色体的拓扑关系。

Topological relationship of prophage lambda to the bacterial chromosome in lysogenic cells.

作者信息

Freifelder D, Meselson M

出版信息

Proc Natl Acad Sci U S A. 1970 Jan;65(1):200-5. doi: 10.1073/pnas.65.1.200.

Abstract

X irradiation converts bacterial sex factor DNA covalent circles to more slowly sedimenting forms according to first order kinetics. The rate of conversion is greater for sex factors carrying lambda prophage than it is for nonlysogenized sex factors. The magnitude of the difference and the absence of covalent circular DNA from the product show that the prophage DNA is linearly inserted into the sex factor, as predicted by Campbell's model for lysogenization.

摘要

X射线照射根据一级动力学将细菌性因子DNA共价环转化为沉降速度更慢的形式。携带λ原噬菌体的性因子的转化速率比未溶源化的性因子更高。差异的大小以及产物中不存在共价环状DNA表明,原噬菌体DNA如坎贝尔溶源化模型所预测的那样线性插入到性因子中。

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Structural change of DNA during lambda-phage lysogenization and induction.
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本文引用的文献

3
Reciprocal recombination in prophage lambda.原噬菌体λ中的交互重组
J Cell Physiol. 1967 Oct;70(2):Suppl:113-8. doi: 10.1002/jcp.1040700409.
9
The twisted circular form of polyoma viral DNA.多瘤病毒DNA的扭曲环状形式。
Proc Natl Acad Sci U S A. 1965 May;53(5):1104-11. doi: 10.1073/pnas.53.5.1104.

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