Freifelder D, Meselson M
Proc Natl Acad Sci U S A. 1970 Jan;65(1):200-5. doi: 10.1073/pnas.65.1.200.
X irradiation converts bacterial sex factor DNA covalent circles to more slowly sedimenting forms according to first order kinetics. The rate of conversion is greater for sex factors carrying lambda prophage than it is for nonlysogenized sex factors. The magnitude of the difference and the absence of covalent circular DNA from the product show that the prophage DNA is linearly inserted into the sex factor, as predicted by Campbell's model for lysogenization.
X射线照射根据一级动力学将细菌性因子DNA共价环转化为沉降速度更慢的形式。携带λ原噬菌体的性因子的转化速率比未溶源化的性因子更高。差异的大小以及产物中不存在共价环状DNA表明,原噬菌体DNA如坎贝尔溶源化模型所预测的那样线性插入到性因子中。