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1
Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles.大肠杆菌性因子的研究:细胞内存在共价环的证据及共价环分离的一般问题。
J Bacteriol. 1971 Mar;105(3):722-7. doi: 10.1128/jb.105.3.722-727.1971.
2
Isolation of minicircular deoxyribonucleic acids from wild strains of Escherichia coli and their relationship to other bacterial plasmids.从大肠杆菌野生菌株中分离微小环状脱氧核糖核酸及其与其他细菌质粒的关系。
J Bacteriol. 1972 Sep;111(3):696-704. doi: 10.1128/jb.111.3.696-704.1972.
3
Isolation and characterization of supercoiled circular deoxyribonucleic acid from beta-hemolytic strains of Escherichia coli.从大肠杆菌β-溶血菌株中分离和鉴定超螺旋环状脱氧核糖核酸
J Bacteriol. 1971 May;106(2):311-7. doi: 10.1128/jb.106.2.311-317.1971.
4
Properties of sex factor and related episomes isolated from purified Escherichia coli zygote cells.从纯化的大肠杆菌合子细胞中分离出的性因子及相关附加体的特性。
J Mol Biol. 1968 Nov 28;38(1):89-108. doi: 10.1016/0022-2836(68)90130-7.
5
Isolation of circular deoxyribonucleic acid from Salmonella typhosa hybrids obtained from matings with Escherichia coli Hfr donors.从与大肠杆菌高频重组(Hfr)供体交配获得的鼠伤寒沙门氏菌杂种中分离环状脱氧核糖核酸。
J Bacteriol. 1971 Dec;108(3):1357-65. doi: 10.1128/jb.108.3.1357-1365.1971.
6
Differences between Escherichia coli B-r and Salmonella typhimurium LT-2 in the sedimentation behavior of the deoxyribonucleic acid synthesized after x-irradiation.X射线照射后合成的脱氧核糖核酸在沉降行为上,大肠杆菌B-r与鼠伤寒沙门氏菌LT-2之间的差异。
J Bacteriol. 1971 Jun;106(3):1043-6. doi: 10.1128/jb.106.3.1043-1046.1971.
7
Anomalies in the sedimentation of deoxyribonucleic acid from Haemophilus influenzae in alkaline sucrose gradients.流感嗜血杆菌脱氧核糖核酸在碱性蔗糖梯度中的沉降异常。
J Bacteriol. 1972 Dec;112(3):1264-9. doi: 10.1128/jb.112.3.1264-1269.1972.
8
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.氯霉素存在下大肠杆菌中Col E 1质粒复制的性质
J Bacteriol. 1972 May;110(2):667-76. doi: 10.1128/jb.110.2.667-676.1972.
9
Influence of thymine starvation on the integrity of deoxyribonucleic acid in Escherichia coli.胸腺嘧啶饥饿对大肠杆菌中脱氧核糖核酸完整性的影响。
J Bacteriol. 1971 Mar;105(3):733-8. doi: 10.1128/jb.105.3.733-738.1971.
10
Studies with Escherichia coli sex factors.关于大肠杆菌性因子的研究。
Cold Spring Harb Symp Quant Biol. 1968;33:425-34. doi: 10.1101/sqb.1968.033.01.049.

引用本文的文献

1
Suppression of Escherichia coli dnaA46 mutations by integration of plasmid R100.1. derivatives: constraints imposed by the replication terminus.通过质粒R100.1衍生物的整合抑制大肠杆菌dnaA46突变:复制终点施加的限制
J Bacteriol. 1982 Aug;151(2):657-67. doi: 10.1128/jb.151.2.657-667.1982.
2
Isolation and characterization of plasmid-deletion derivatives from an Hfr made with Rts1 plasmid carrying a chromosomal mutation, plt.从携带染色体突变plt的Rts1质粒构建的高频重组菌(Hfr)中分离并鉴定质粒缺失衍生物。
Mol Gen Genet. 1980 Jan;177(2):243-50. doi: 10.1007/BF00267435.
3
Plasmid ColVBtrp maintenance in Erwinia carotovora.胡萝卜软腐欧文氏菌中质粒ColVBtrp的维持
J Bacteriol. 1981 Sep;147(3):1015-20. doi: 10.1128/jb.147.3.1015-1020.1981.
4
A potential procedure for obtaining mutants of Escherichia coli that affect plasmid stability.一种获得影响质粒稳定性的大肠杆菌突变体的潜在方法。
Mol Gen Genet. 1974;133(2):105-10. doi: 10.1007/BF00264831.
5
Molecular nature of the deoxyribonucleic acid of a thermosensitive R factor, Rts1.温度敏感型R因子Rts1的脱氧核糖核酸的分子性质
J Bacteriol. 1974 Dec;120(3):1364-9. doi: 10.1128/jb.120.3.1364-1369.1974.
6
The thermosensitive lesion in the replication of the drug resistance factor, Rts1.耐药因子Rts1复制中的热敏性损伤。
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2515-9. doi: 10.1073/pnas.71.6.2515.
7
Round of replication mutant of a drug resistance factor.耐药因子的一轮复制突变体。
J Bacteriol. 1974 Jun;118(3):855-66. doi: 10.1128/jb.118.3.855-866.1974.
8
Mapping loci for surface exclusion and incompatibility on the F factor of Escherichia coli K-12.定位大肠杆菌K-12 F因子上表面排斥和不相容性的基因座。
J Bacteriol. 1974 Jun;118(3):778-82. doi: 10.1128/jb.118.3.778-782.1974.
9
A simplified method for preparing sucrose gradients.一种制备蔗糖梯度的简化方法。
Biochem J. 1974 Jan;137(1):117-8. doi: 10.1042/bj1370117.
10
Two major groups of colicin factors: their evolutionary significance.大肠杆菌素因子的两大主要类别:它们的进化意义
Mol Gen Genet. 1973 Sep 12;125(3):217-30. doi: 10.1007/BF00270744.

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SYNTHESIS OF BETA-D-GALACTOSIDASE AFTER F-DUCTION OF LAC+GENES INTO ESCHERICHIA COLI.将乳糖操纵子基因导入大肠杆菌后β-D-半乳糖苷酶的合成
J Mol Biol. 1965 Jan;11:23-34. doi: 10.1016/s0022-2836(65)80168-1.
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THE CYCLIC HELIX AND CYCLIC COIL FORMS OF POLYOMA VIRAL DNA.多瘤病毒DNA的环状螺旋和环状盘绕形式
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Physicochemical studies on the reaction between formaldehyde and DNA.甲醛与DNA反应的物理化学研究。
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Circular dimer and catenate forms of mitochondrial DNA in human leukaemic leucocytes.人白血病白细胞中线粒体DNA的环状二聚体和连环体形式
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Composite circular forms of R factor deoxyribonucleic acid molecules.R 因子脱氧核糖核酸分子的复合环状形式
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The steric effect in lysogenization by bacteriophage lambda. I. Lysogenization of a partially diploid strain of Escherichia coli K-12.λ噬菌体溶原化过程中的空间效应。I. 大肠杆菌K-12部分二倍体菌株的溶原化
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Circular DNA forms of a bacterial sex factor.细菌性因子的环状DNA形式。
Proc Natl Acad Sci U S A. 1967 Oct;58(4):1731-8. doi: 10.1073/pnas.58.4.1731.
8
Evidence for circular DNA forms of a bacterial plasmid.细菌质粒环状DNA形式的证据。
Proc Natl Acad Sci U S A. 1967 Aug;58(2):650-7. doi: 10.1073/pnas.58.2.650.
9
Studies on Escherichia coli sex factors. IV. Molecular weights of the DNA of several F' elements.大肠杆菌性因子的研究。IV. 几种F' 因子DNA的分子量
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10
Studies on Escherichia coli sex factors. 3. Covalently closed F'Lac DNA molecules.大肠杆菌性别因子的研究。3. 共价闭合的F'Lac DNA分子。
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大肠杆菌性因子的研究:细胞内存在共价环的证据及共价环分离的一般问题。

Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles.

作者信息

Freifelder D, Folkmanis A, Kirschner I

出版信息

J Bacteriol. 1971 Mar;105(3):722-7. doi: 10.1128/jb.105.3.722-727.1971.

DOI:10.1128/jb.105.3.722-727.1971
PMID:4926680
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC248492/
Abstract

We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The causes of the culture-to-culture variation in recovery of covalent circular deoxyribonucleic acid were investigated but an understanding of this is not yet at hand. Some commonly used conditions which drastically reduce the recovery of covalent circles are described.

摘要

我们详细研究了从含有性因子的细胞中以共价环形式获得可重复且最大量脱氧核糖核酸所需的条件。结果表明,在最佳条件下,共价环在分离过程中既不会产生也不会丢失。我们研究了不同培养物中共价环状脱氧核糖核酸回收率存在差异的原因,但目前对此还尚未完全了解。文中还描述了一些会大幅降低共价环回收率的常用条件。