大肠杆菌性因子的研究：细胞内存在共价环的证据及共价环分离的一般问题。
Studies on Escherichia coli sex factors: evidence that covalent circles exist within cells and the general problem of isolation of covalent circles.
作者信息
Freifelder D, Folkmanis A, Kirschner I
出版信息
J Bacteriol. 1971 Mar;105(3):722-7. doi: 10.1128/jb.105.3.722-727.1971.
Abstract
We examined in detail conditions necessary for making reproducible and for maximizing the amount of deoxyribonucleic acid obtained from a sex factor-containing cell as covalent circles. The results argue that under optimal conditions covalent circles are neither created nor lost during the isolation procedure. The causes of the culture-to-culture variation in recovery of covalent circular deoxyribonucleic acid were investigated but an understanding of this is not yet at hand. Some commonly used conditions which drastically reduce the recovery of covalent circles are described.
摘要
我们详细研究了从含有性因子的细胞中以共价环形式获得可重复且最大量脱氧核糖核酸所需的条件。结果表明，在最佳条件下，共价环在分离过程中既不会产生也不会丢失。我们研究了不同培养物中共价环状脱氧核糖核酸回收率存在差异的原因，但目前对此还尚未完全了解。文中还描述了一些会大幅降低共价环回收率的常用条件。
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