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无血清和添加小鼠血清的培养基中小鼠细胞介导的淋巴细胞溶解试验：培养条件和遗传因素

Mouse cell-mediated lympholysis assay in serum-free and mouse serum-supplemented media: culture conditions and genetic factors.

作者信息

Peck A B, Bach F H

出版信息

Scand J Immunol. 1975;4(1):53-62. doi: 10.1111/j.1365-3083.1975.tb02599.x.

DOI:10.1111/j.1365-3083.1975.tb02599.x
PMID:49078
Abstract

We describe a miniaturized cell-mediated lympholysis assay for measuring the activity of cytotoxic lymphocytes generated in mouse mixed leukocyte cultures (MLC), using either serum-free or mouse serum-supplemented media. Mixed reactions of strain combinations possessing various genetic differences at H-2 produced cytotoxic lymphocytes with cytolytic activity against cells of the donor used in the sensitization phase. Results suggest that both serologically defined and lymphocyte-defined determinants can act as effective target antigens on target cells. In contrast, no cytotoxic lymphocytes were generated against products of non-H-2 loci--M-locus, theta, or other non-H-2 antigens--that induce MLC activation.

摘要

我们描述了一种小型化的细胞介导的淋巴细胞溶解试验，用于测量在小鼠混合淋巴细胞培养物（MLC）中产生的细胞毒性淋巴细胞的活性，该试验使用无血清培养基或添加小鼠血清的培养基。在H-2位点具有各种遗传差异的品系组合的混合反应产生了对致敏阶段所用供体细胞具有溶细胞活性的细胞毒性淋巴细胞。结果表明，血清学定义的和淋巴细胞定义的决定簇均可作为靶细胞上的有效靶抗原。相比之下，未产生针对诱导MLC激活的非H-2基因座产物（M-基因座、θ或其他非H-2抗原）的细胞毒性淋巴细胞。

相似文献

1
Mouse cell-mediated lympholysis assay in serum-free and mouse serum-supplemented media: culture conditions and genetic factors.无血清和添加小鼠血清的培养基中小鼠细胞介导的淋巴细胞溶解试验：培养条件和遗传因素
Scand J Immunol. 1975;4(1):53-62. doi: 10.1111/j.1365-3083.1975.tb02599.x.
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Specificity of cell-mediated lympholysis for public and private H-2 determinants.细胞介导的淋巴细胞溶解对公共和私有H-2决定簇的特异性
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引用本文的文献

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Generation of primary cytotoxic lymphocytes against non-major histocompatibility complex antigens by anti-Ia serum plus complement-treated lymphocytes.通过抗Ia血清加补体处理的淋巴细胞产生针对非主要组织相容性复合体抗原的原发性细胞毒性淋巴细胞。
J Exp Med. 1980 May 1;151(5):1305-10. doi: 10.1084/jem.151.5.1305.
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Activation of natural killer (NK) cells in vivo with H-2 and non-H-2 alloantigens.用H-2和非H-2同种异体抗原在体内激活自然杀伤（NK）细胞。
Immunogenetics. 1981;12(3-4):221-35. doi: 10.1007/BF01561666.
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Minor histocompatibility antigen expression on F9 embryonal carcinoma cells revealed by T-cell mediated responses.
通过T细胞介导的反应揭示F9胚胎癌细胞上的次要组织相容性抗原表达。
Immunogenetics. 1982;16(4):349-54. doi: 10.1007/BF00372306.
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Alloreactive cloned T cell lines. I. Interactions between cloned amplifier and cytolytic T cell lines.同种异体反应性克隆T细胞系。I. 克隆扩增性T细胞系与细胞溶解性T细胞系之间的相互作用。
J Exp Med. 1980 Apr 1;151(4):876-95. doi: 10.1084/jem.151.4.876.
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Primary T-cell responses to minor alloantigens. II. Analysis of accessory cell requirements for the development of cytotoxic T lymphocytes.针对次要同种异体抗原的初始T细胞应答。II. 细胞毒性T淋巴细胞发育所需辅助细胞的分析。
Immunology. 1983 Dec;50(4):555-63.
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Lymphocyte chemotaxis in inflammation. VI. Lyt phenotype analysis of effector cells responsible for producing murine lymphocyte chemotactic factor.炎症中的淋巴细胞趋化作用。VI. 负责产生小鼠淋巴细胞趋化因子的效应细胞的Lyt表型分析。
Immunology. 1983 May;49(1):95-102.
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Suppressor T cells activated in a primary in vitro response to non-major histocompatibility alloantigens.在对非主要组织相容性同种异体抗原的初次体外应答中被激活的抑制性T细胞。
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I. Characterization of cytotoxic effector cells generated from regional lymph nodes after immunization in the footpad.一、足垫免疫后区域淋巴结产生的细胞毒性效应细胞的特征
Immunology. 1983 Sep;50(1):121-9.
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