Olivera B M, Bonhoeffer F
Proc Natl Acad Sci U S A. 1972 Jan;69(1):25-9. doi: 10.1073/pnas.69.1.25.
Lysates of an Escherichia coli polA(-) strain convert single-stranded DNA from varphiX174 virus to the double-stranded replicative form with high efficiency on cellophane discs. The initiation of synthesis of the complementary strand appears to be rate limiting; once initiation occurs, the chain is propagated rapidly. Under these conditions, the unsealed replicative form accumulates and is slowly converted to the sealed form; this conversion requires the activity of the DPN-dependent DNA ligase.
大肠杆菌polA(-)菌株的裂解物能在玻璃纸圆盘上高效地将来自φX174病毒的单链DNA转化为双链复制型。互补链合成的起始似乎是限速步骤；一旦起始发生，链的延伸就很快。在这些条件下，未封口的复制型会积累并缓慢转化为封口形式；这种转化需要依赖DPN的DNA连接酶的活性。
