由依赖DNA的RNA聚合酶起始并释放RNA
Initiation and release of RNA by DNA-dependent RNA polymerase.
作者信息
Millette R L, Trotter C D
出版信息
Proc Natl Acad Sci U S A. 1970 Jul;66(3):701-8. doi: 10.1073/pnas.66.3.701.
Abstract
During in vitro transcription of T4 DNA by E. coli RNA polymerase, chain initiation stops coincidentally with synthesis at low ionic strength (0.11) with an average of one RNA chain initiated per 24S polymerase molecule. At high ionic strength (0.37), initiation as well as synthesis continues for several hours, with an average of four chains initiated per enzyme molecule in two hours. The RNA product is released from the T4 DNA template at both low and high ionic strength. At high ionic strength, however, RNA polymerase can repeatedly initiate, synthesize, and release RNA from the synthesis complex in vitro. Under both conditions, the synthesized RNA sediments at 25-44 S, has a number average chain length of 5000 to 7500 nucleotides, and a weight average chain length of 11,500 nucleotides.
摘要
在大肠杆菌RNA聚合酶对T4 DNA进行体外转录时，在低离子强度（0.11）下，链起始与合成同时停止，平均每24S聚合酶分子起始一条RNA链。在高离子强度（0.37）下，起始以及合成会持续数小时，两小时内平均每个酶分子起始四条链。在低离子强度和高离子强度下，RNA产物均从T4 DNA模板上释放。然而，在高离子强度下，RNA聚合酶能够在体外从合成复合物中反复起始、合成并释放RNA。在两种条件下，合成的RNA在25 - 44 S沉降，数均链长为5000至7500个核苷酸，重均链长为11,500个核苷酸。
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