Williams G, Newberne P M
Br J Exp Pathol. 1970 Jun;51(3):253-64.
Five month old male beagles injected i.p. with a washed 18-hr culture of 1 × 10 organisms/kg. responded clinically with a marked depression, anorexia, and a febrile response 12 hr following infection. Animals allowed to live beyond 36 hr showed a decrease in febrile response. Although they still refused food 36 hr following infection, they appeared more alert by 60 hr post-infection. Changes at necropsy were minimal and histologic findings consistent with Salmonella infection were found in both liver and spleen. In animals infected 60 hr liver microabscesses and circumscribed foci of reticuloendothelial cell hyperplasia in the spleen indicated that the animals were responding favourably by mounting a defence against the infection. As a result of infection there was an outpouring of band neutrophils resulting in an increase in the absolute numbers of circulating white blood cells. Associated with the neutrophilia was an early lymphopenia and eosinopenia. The total free amino acids were depressed in serum and increased in the liver at all time periods observed, but the degree of change as well as the specific amino acids affected varied; however, alterations in concentration of amino acids in serum of animals infected with Salmonella were similar to those observed in dogs infected with the virus of canine distemper. In both viral and bacterial infected dogs serum phenylalanine was increased and tyrosine remained the same or decreased, resulting in a significant alteration in the phenylalanine/tyrosine (P/T) ratio. As with distemper infected dogs, an increase of the alpha-2 fraction of serum proteins was detected at 36 hr following infection. There was a decrease in the monosome peak and a corresponding increase in the polysome fraction of the liver post-mitochondrial supernatant preparation associated with infection. The significance of this change in the liver polysome profile following infection is not yet understood.
5 月龄雄性比格犬经腹腔注射每千克体重1×10个经过洗涤的18小时培养物。感染后12小时，动物出现明显的抑郁、厌食和发热反应。存活超过36小时的动物发热反应有所减轻。虽然感染后36小时它们仍然拒食，但感染后60小时它们显得更加警觉。尸检时变化极小，在肝脏和脾脏中均发现了与沙门氏菌感染一致的组织学表现。在感染60小时的动物中，肝脏微脓肿和脾脏中网状内皮细胞增生的局限性病灶表明动物通过对感染进行防御而产生了良好的反应。感染导致带状中性粒细胞增多，从而使循环白细胞的绝对数量增加。与嗜中性粒细胞增多相关的是早期淋巴细胞减少和嗜酸性粒细胞减少。在观察的所有时间段内，血清中总游离氨基酸水平降低，肝脏中总游离氨基酸水平升高，但变化程度以及受影响的特定氨基酸各不相同；然而，感染沙门氏菌的动物血清中氨基酸浓度的变化与感染犬瘟热病毒的犬相似。在病毒感染和细菌感染的犬中，血清苯丙氨酸均升高，酪氨酸保持不变或降低，导致苯丙氨酸/酪氨酸（P/T）比值发生显著变化。与感染犬瘟热的犬一样，感染后36小时检测到血清蛋白的α-2 组分增加。感染后，肝脏线粒体后上清液制备物中的单体峰减少，多核糖体组分相应增加。感染后肝脏多核糖体谱的这种变化的意义尚不清楚。
