Schmassmann A, Mikuz G, Bartsch G, Rohr H
Microsc Acta. 1979 Sep;82(2):163-78.
Sperm morphology and motility are determined using a semi-automatic image analysis system under opto-manual control. The system consists of a microscope equipped with a drawing-tube, a digitizer-tablet, and a cursor interfaced to a small microcomputer. A light-emitting diode is mounted on the cursor, visible as a bright red pilot-light in the microscopic field. Sperm head and midpiece as well as the pathway of motile spermatozoa are traced with the cursor's red pilot light. The microcomputer calculates area, circumference, and length simultaneously. Motility and sperm density are determined altogether using microchamber technique. In a selected group of male subjects with normal, doubtful, and pathological semen analyses biometrical analyses of sperm morphology and motility were performed. Sperm morphology is best described by the variation of the head size and by the average midpiece width. Motility is best described by % motile spermatozoa and mean velocity determined 1 hour after semen collection. Biometrical semen analyses are superior to subjective evaluations regarding degree of information, objectivity, and reproducibility.
精子形态和活力通过半自动图像分析系统在光学手动控制下进行测定。该系统由一台配备绘图管的显微镜、一个数字化仪平板和一个与小型微型计算机相连的光标组成。光标上安装有一个发光二极管，在显微镜视野中可见为亮红色指示灯。用光标上的红色指示灯追踪精子头部、中段以及活动精子的路径。微型计算机同时计算面积、周长和长度。活力和精子密度使用微室技术一并测定。在一组精液分析正常、可疑和病理性的选定男性受试者中，对精子形态和活力进行了生物测量分析。精子形态最好通过头部大小的变化和中段平均宽度来描述。活力最好通过精液采集后1小时测定的活动精子百分比和平均速度来描述。生物测量精液分析在信息程度、客观性和可重复性方面优于主观评估。
