Makler A, Tatcher M, Mohilever J
Int J Fertil. 1980;25(1):62-6.
A semiautomatic method for sperm analysis is described. The method combines a previously developed multiple exposure photography system with a digitizer connected to a computer. An undiluted specimen is inserted into a 10-mic chamber and the sperms are microscopically photographed for 1 second while illuminated by six short light pulses. On the developed film nonmotile sperms appear very bright, while motile sperms are seen as six-ringed chains. In addition, high magnification photographs of immobilized preheated sperms are taken for morphology analysis. The photographed images are projected onto sheets of paper where the traces of the nonmotile and motile sperms as well as the normal and abnormal forms are marked by a pen. For motility determination, sheets containing the sperm tracks are placed on the digitizer tablet. By means of a sonic pen the coordinates of the ends of the tracks are automatically recorded and the number of motile and nonmotile sperms are counted. Then the percent of abnormal forms are entered at the keyboard. Within a few seconds the computer supplies a printout of the results, including sperm concentration, percent of motile sperms, average velocity and frequency distribution of velocities of moving sperms, percent of abnormal forms, and total number of analyzed sperms.
本文描述了一种用于精子分析的半自动方法。该方法将先前开发的多重曝光摄影系统与连接到计算机的数字化仪相结合。将未稀释的样本放入10微米的腔室中，在六个短光脉冲照射下，对精子进行1秒钟的显微镜拍摄。在显影后的胶片上，不活动的精子显得非常明亮，而活动的精子则呈现为六环链状。此外，还拍摄固定预热精子的高倍照片用于形态分析。拍摄的图像投影到纸张上，用笔画出不活动和活动精子的轨迹以及正常和异常形态。为了测定活力，将包含精子轨迹的纸张放在数字化仪平板上。通过声波笔自动记录轨迹末端的坐标，并计算活动和不活动精子的数量。然后在键盘上输入异常形态的百分比。几秒钟内，计算机就会提供结果的打印输出，包括精子浓度、活动精子百分比、平均速度和活动精子速度的频率分布、异常形态百分比以及分析的精子总数。
