Hartman K A, Thomas G J
Science. 1970 Nov 13;170(3959):740-1. doi: 10.1126/science.170.3959.740.
Infrared spectra were obtained for 16S and for 23S ribosomal RNA's in D(2)O solutions. The percentage of each base in the paired and unpaired regions of the RNA was determined from the spectra. The secondary structures of 16S and 23S ribosomal RNA's (from Escherichia coli) are significantly different from each other and are also different from those of yeast ribosomal RNA, formylmethionyl-transfer RNA, and the anticodon fragment of this transfer RNA.
在重水(D₂O)溶液中获得了16S和23S核糖体RNA的红外光谱。从光谱中确定了RNA配对区和非配对区中每种碱基的百分比。(来自大肠杆菌的)16S和23S核糖体RNA的二级结构彼此显著不同,也与酵母核糖体RNA、甲酰甲硫氨酰 - 转移RNA以及该转移RNA的反密码子片段的二级结构不同。