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大肠杆菌核糖体tRNA结合位点处的蛋白质。

Proteins at the tRNA binding sites of Escherichia coli ribosomes.

作者信息

Czernilofsky A P, Collatz E E, Stöffler G, Kuechler E

出版信息

Proc Natl Acad Sci U S A. 1974 Jan;71(1):230-4. doi: 10.1073/pnas.71.1.230.

DOI:10.1073/pnas.71.1.230
PMID:4589893
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC387971/
Abstract

p - Nitrophenylcarbamyl - phenylalanyl-tRNA binds to ribosomes in response to poly(U) and reacts with ribosomal proteins via covalent bond formation. Ribosomal proteins of Escherichia coli were characterized by two-dimensional gel electrophoresis and by reaction with specific antibodies. The affinity label is shown to react with the 50S proteins L27, L15, L2, L16, and L14. We therefore conclude that these particular proteins are located at or near tRNA-binding sites within the 50S ribosomal subunit.

摘要

对硝基苯基氨基甲酰 - 苯丙氨酰 - tRNA响应聚（U）与核糖体结合，并通过共价键形成与核糖体蛋白反应。通过二维凝胶电泳和与特异性抗体反应对大肠杆菌的核糖体蛋白进行了表征。结果表明，亲和标记物与50S蛋白L27、L15、L2、L16和L14发生反应。因此，我们得出结论，这些特定的蛋白质位于50S核糖体亚基内的tRNA结合位点处或其附近。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d72/387971/42bf6af962d3/pnas00054-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d72/387971/42bf6af962d3/pnas00054-0237-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7d72/387971/42bf6af962d3/pnas00054-0237-a.jpg

相似文献

1
Proteins at the tRNA binding sites of Escherichia coli ribosomes.大肠杆菌核糖体tRNA结合位点处的蛋白质。
Proc Natl Acad Sci U S A. 1974 Jan;71(1):230-4. doi: 10.1073/pnas.71.1.230.
2
Identification of 50S proteins at the peptidyl-tRNA binding site of Escherichia coli ribosomes.大肠杆菌核糖体肽基 - tRNA结合位点处50S蛋白的鉴定。
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Peptidyl transferase inhibitors alter the covalent reaction of BrAcPhe-tRNA with the E. coli ribosome.肽基转移酶抑制剂会改变溴乙酰苯丙氨酰 - tRNA与大肠杆菌核糖体的共价反应。
FEBS Lett. 1974 Sep 1;45(1):218-22. doi: 10.1016/0014-5793(74)80848-3.

引用本文的文献

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A new simpler photoaffinity analogue of peptidyl tRNA.一种新型的更简单的肽基tRNA光亲和类似物。
Nucleic Acids Res. 1974 Dec;1(12):1753-62. doi: 10.1093/nar/1.12.1753.
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Photo-affinity labeling of tRNA binding sites in macromolecules. I. Linking of the phenacyl-p-azide of 4-thiouridine in (Escherichia coli) valyl-tRNA to 16S RNA at the ribosomal P site.大分子中tRNA结合位点的光亲和标记。I.（大肠杆菌）缬氨酰 - tRNA中4 - 硫尿苷的苯甲酰基 - 叠氮化物与核糖体P位点的16S RNA的连接
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3951-5. doi: 10.1073/pnas.71.10.3951.
3
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本文引用的文献

1
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.一种测定酶沉降行为的方法：应用于蛋白质混合物
J Biol Chem. 1961 May;236:1372-9.
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Irreversible binding of chloramphenicol analogues to E. coli ribosomes.氯霉素类似物与大肠杆菌核糖体的不可逆结合。
FEBS Lett. 1972 Dec 1;28(2):149-152. doi: 10.1016/0014-5793(72)80698-7.
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Synthesis of tRNA ureido derivatives as substrates for The invention of the ribosome peptidyl transferase center.作为核糖体肽基转移酶中心发明底物的tRNA脲基衍生物的合成。
一种将GTP的γ-磷酸基团转移至真核生物40S核糖体亚基蛋白质上的激酶。
Proc Natl Acad Sci U S A. 1974 Feb;71(2):350-4. doi: 10.1073/pnas.71.2.350.
4
Localization of ribosomal protein L27 at the peptidyl transferase centre of the 50 S subunit, as determined by immuno-electron microscopy.通过免疫电子显微镜确定核糖体蛋白L27在50 S亚基肽基转移酶中心的定位。
Mol Gen Genet. 1987 Dec;210(3):498-503. doi: 10.1007/BF00327203.
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Mutational study of the rRNA in yeast mitochondria: functional importance of T1696 in the large rRNA gene.酵母线粒体中rRNA的突变研究：大rRNA基因中T1696的功能重要性。
Nucleic Acids Res. 1988 Oct 11;16(19):9299-306. doi: 10.1093/nar/16.19.9299.
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A model for the spatial arrangement of the proteins in the large subunit of the Escherichia coli ribosome.大肠杆菌核糖体大亚基中蛋白质空间排列的模型。
EMBO J. 1988 Nov;7(11):3571-6. doi: 10.1002/j.1460-2075.1988.tb03234.x.
7
Photoincorporation of puromycin and N-(ethyl-2-diazomalonyl)puromycin into Escherichia coli ribosomes.嘌呤霉素和N-（乙基-2-重氮丙二酰基）嘌呤霉素光掺入大肠杆菌核糖体。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):2974-8. doi: 10.1073/pnas.72.8.2974.
8
Affinity labeling of the ribosomal decoding site with an AUG-substrate analog.用AUG底物类似物对核糖体解码位点进行亲和标记。
Proc Natl Acad Sci U S A. 1975 Apr;72(4):1505-9. doi: 10.1073/pnas.72.4.1505.
9
Identification of a ribosomal protein essential for peptidyl transferase activity.鉴定一种对肽基转移酶活性至关重要的核糖体蛋白。
Proc Natl Acad Sci U S A. 1975 Mar;72(3):844-8. doi: 10.1073/pnas.72.3.844.
10
Identification of components of the streptomycin-binding center of E. coli MRE 600 ribosomes by photo-affinity labelling.通过光亲和标记鉴定大肠杆菌MRE 600核糖体链霉素结合中心的成分
Mol Gen Genet. 1976 Mar 22;144(2):205-12. doi: 10.1007/BF02428110.
FEBS Lett. 1972 Aug 1;24(2):201-203. doi: 10.1016/0014-5793(72)80767-1.
4
Studies on the formation of transfer ribonucleic acid-ribosome complexes. 8. Aminoacyl oligonucleotide binding to ribosomes: characteristics and requirements.转移核糖核酸-核糖体复合物形成的研究。8. 氨酰寡核苷酸与核糖体的结合：特性与要求。
J Biol Chem. 1970 Nov 25;245(22):6208-19.
5
Polypeptide chain initiation: nucleotide sequences of the three ribosomal binding sites in bacteriophage R17 RNA.多肽链起始：噬菌体R17 RNA中三个核糖体结合位点的核苷酸序列
Nature. 1969 Dec 6;224(5223):957-64. doi: 10.1038/224957a0.
6
Studies on the formation of transfer ribonucleic acid-ribosome complexes. XI. Antibiotic effects on phenylalanyl-oligonucleotide binding to ribosomes.转移核糖核酸-核糖体复合物形成的研究。XI. 抗生素对苯丙氨酰寡核苷酸与核糖体结合的影响。
Proc Natl Acad Sci U S A. 1969 Oct;64(2):709-14. doi: 10.1073/pnas.64.2.709.
7
Ribosomal proteins. XXV. Immunological studies on Escherichia coli ribosomal proteins.核糖体蛋白。二十五。大肠杆菌核糖体蛋白的免疫学研究。
J Mol Biol. 1971 Dec 14;62(2):407-9. doi: 10.1016/0022-2836(71)90436-0.
8
Sequence differences of Escherichia coli 30S ribosomal proteins as determined by immunochemical methods.用免疫化学方法测定的大肠杆菌30S核糖体蛋白的序列差异。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2283-7. doi: 10.1073/pnas.68.9.2283.
9
Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis.核糖体蛋白。十二。通过二维凝胶电泳测定大肠杆菌小核糖体亚基和大核糖体亚基中的蛋白数量。
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1276-82. doi: 10.1073/pnas.67.3.1276.
10
Chemical studies on methionyl-tRNA synthetase from Escherichia coli.大肠杆菌甲硫氨酰 - tRNA合成酶的化学研究。
J Mol Biol. 1970 Sep 14;52(2):165-78. doi: 10.1016/0022-2836(70)90023-9.