大肠杆菌核糖体tRNA结合位点处的蛋白质。
Proteins at the tRNA binding sites of Escherichia coli ribosomes.
作者信息
Czernilofsky A P, Collatz E E, Stöffler G, Kuechler E
出版信息
Proc Natl Acad Sci U S A. 1974 Jan;71(1):230-4. doi: 10.1073/pnas.71.1.230.
p - Nitrophenylcarbamyl - phenylalanyl-tRNA binds to ribosomes in response to poly(U) and reacts with ribosomal proteins via covalent bond formation. Ribosomal proteins of Escherichia coli were characterized by two-dimensional gel electrophoresis and by reaction with specific antibodies. The affinity label is shown to react with the 50S proteins L27, L15, L2, L16, and L14. We therefore conclude that these particular proteins are located at or near tRNA-binding sites within the 50S ribosomal subunit.
对硝基苯基氨基甲酰 - 苯丙氨酰 - tRNA响应聚（U）与核糖体结合，并通过共价键形成与核糖体蛋白反应。通过二维凝胶电泳和与特异性抗体反应对大肠杆菌的核糖体蛋白进行了表征。结果表明，亲和标记物与50S蛋白L27、L15、L2、L16和L14发生反应。因此，我们得出结论，这些特定的蛋白质位于50S核糖体亚基内的tRNA结合位点处或其附近。
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