大肠杆菌的DNA连接酶突变体
DNA ligase mutants of Escherichia coli.
作者信息
Gellert M, Bullock M L
出版信息
Proc Natl Acad Sci U S A. 1970 Nov;67(3):1580-7. doi: 10.1073/pnas.67.3.1580.
Abstract
A procedure is described for the isolation of Escherichia coli mutants with either excess or deficient DNA ligase activity. A mutant that overproduces DNA ligase supports the growth of ligase-defective (gene 30 mutant) T4 phages. Even T4 rII-gene 30 double mutants, which are able to grow in normal E. coli, cannot grow in cells deficient in DNA ligase. A functional DNA ligase, supplied either by the phage or the host, thus seems to be required for T4 growth. An E. coli strain that makes a temperature-sensitive DNA ligase becomes radiation-sensitive at high temperature, but otherwise grows normally and shows no obvious defect in DNA replication.
摘要
本文描述了一种分离具有过量或缺陷DNA连接酶活性的大肠杆菌突变体的方法。一个过量产生DNA连接酶的突变体能够支持连接酶缺陷型（基因30突变体）T4噬菌体的生长。即使是能够在正常大肠杆菌中生长的T4 rII - 基因30双突变体，也不能在DNA连接酶缺陷的细胞中生长。因此，无论是由噬菌体还是宿主提供的功能性DNA连接酶，似乎都是T4生长所必需的。一种产生温度敏感型DNA连接酶的大肠杆菌菌株在高温下对辐射敏感，但在其他方面生长正常，并且在DNA复制中没有明显缺陷。
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本文引用的文献
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CHROMOSOME STRUCTURE IN PHAGE T4. I. CIRCULARITY OF THE LINKAGE MAP.噬菌体T4中的染色体结构。I. 连锁图谱的环形性
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Linkage of polynucleotides through phosphodiester bonds by an enzyme from Escherichia coli.来自大肠杆菌的一种酶通过磷酸二酯键连接多核苷酸。
Proc Natl Acad Sci U S A. 1967 May;57(5):1426-33. doi: 10.1073/pnas.57.5.1426.
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Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.脱氧核糖核酸的酶促断裂与连接，I. 用感染T4噬菌体的大肠杆菌的酶系统修复DNA中的单链断裂
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Suppression of T4D ligase mutations by rIIa and rIIb mutations.rIIa和rIIb突变对T4D连接酶突变的抑制作用。
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10
DNA replication in vivo by a temperature-sensitive polynucleotide ligase mutant of T4.利用T4的温度敏感型多核苷酸连接酶突变体在体内进行DNA复制。
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