酿酒酵母的cdc9连接酶缺陷型突变体对博来霉素的致死效应表现出较低的抗性。
cdc9 ligase-defective mutants of Saccharomyces cerevisiae exhibit lowered resistance to lethal effects of bleomycin.
作者信息
Moore C W
出版信息
J Bacteriol. 1982 Sep;151(3):1617-20. doi: 10.1128/jb.151.3.1617-1620.1982.
Abstract
Conditional ligase-deficient mutants of Saccharomyces cerevisiae were more sensitive than their parental (CDC9) strain to dose-dependent killing by bleomycin, even when mutant cells were pregrown and exposed to the antibiotic at permissive temperatures. Pretreatment incubation at the restrictive temperature (37 degrees C) under growing or nongrowing conditions enhanced bleomycin killing of both cdc9-1 and cdc9-9 mutants. This sensitization could be relieved by incubation at the permissive temperature before treatment.
摘要
酿酒酵母的条件性连接酶缺陷型突变体比其亲本（CDC9）菌株对博来霉素的剂量依赖性杀伤更敏感，即使突变细胞在允许温度下预培养并暴露于抗生素中也是如此。在生长或非生长条件下于限制温度（37摄氏度）进行预处理孵育，可增强博来霉素对cdc9 - 1和cdc9 - 9突变体的杀伤作用。这种敏感性可通过在处理前于允许温度下孵育来缓解。
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本文引用的文献
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Genetic Control of the Cell Division Cycle in Yeast: V. Genetic Analysis of cdc Mutants.酵母细胞分裂周期的遗传控制：V. cdc 突变体的遗传分析。
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J Bacteriol. 1982 Jun;150(3):1227-33. doi: 10.1128/jb.150.3.1227-1233.1982.
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