微生物菌落的自动计数。
Automated counting of microbial colonies.
作者信息
Schoon D J, Drake J F, Fredrickson A G, Tsuchiya H M
出版信息
Appl Microbiol. 1970 Nov;20(5):815-20. doi: 10.1128/am.20.5.815-820.1970.
The time required for direct counting of colonies on agar plates for estimating population density of viable microorganisms has precluded studies requiring measurement of such a parameter. Bowman, Blume, and Vurek have described a photo-electrical capillary tube scanner which automates the counting. Results obtained with an instrument similar to that of Bowman et al. have been intensively analyzed with respect to precision and accuracy. The sources of "errors" have been ascertained, and the instrument's potentialities and limitations are discussed.
通过在琼脂平板上直接计数菌落来估算活微生物种群密度所需的时间，使得那些需要测量该参数的研究无法开展。鲍曼、布卢姆和武雷克描述了一种光电毛细管扫描仪，它能实现计数自动化。对于一台与鲍曼等人所使用的仪器类似的设备所获得的结果，已就其精密度和准确度进行了深入分析。已确定了“误差”来源，并讨论了该仪器的潜力和局限性。
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