Standing C N, Fredrickson A G, Tsuchiya H M
Appl Microbiol. 1972 Feb;23(2):354-9. doi: 10.1128/am.23.2.354-359.1972.
Batch growth of Escherichia coli in the presence of equal initial concentrations of glucose and a secondary substrate (xylose) is characterized by sequential utilization of the substrates, whereas continuous-culture systems with equal concentrations of the two substrates in the feed are characterized by complete utilization of both substrates at both high and low dilution rates. Such systems at steady state at a low dilution rate, when suddenly shifted to a higher dilution rate, experience a transient drop in population density accompanied by accumulation of the secondary substrate but virtually no accumulation of glucose. Systems at steady state with 200 mg of glucose per liter were found to undergo a transient population decrease and eventual recovery when switched to feed containing 200 mg of a secondary substrate per liter.
在初始葡萄糖浓度与第二种底物（木糖）浓度相等的情况下，大肠杆菌的分批培养表现为底物的顺序利用，而在进料中两种底物浓度相等的连续培养系统中，无论稀释率高低，两种底物都会被完全利用。在低稀释率下处于稳态的此类系统，当突然转换到较高稀释率时，会经历种群密度的短暂下降，同时伴随着第二种底物的积累，但几乎没有葡萄糖的积累。研究发现，每升含200毫克葡萄糖的稳态系统，在转换为每升含200毫克第二种底物的进料时，会经历种群数量的短暂减少并最终恢复。
