两种底物系统的分批培养和连续培养瞬变过程。
Batch- and continuous-culture transients for two substrate systems.
作者信息
Standing C N, Fredrickson A G, Tsuchiya H M
出版信息
Appl Microbiol. 1972 Feb;23(2):354-9. doi: 10.1128/am.23.2.354-359.1972.
Abstract
Batch growth of Escherichia coli in the presence of equal initial concentrations of glucose and a secondary substrate (xylose) is characterized by sequential utilization of the substrates, whereas continuous-culture systems with equal concentrations of the two substrates in the feed are characterized by complete utilization of both substrates at both high and low dilution rates. Such systems at steady state at a low dilution rate, when suddenly shifted to a higher dilution rate, experience a transient drop in population density accompanied by accumulation of the secondary substrate but virtually no accumulation of glucose. Systems at steady state with 200 mg of glucose per liter were found to undergo a transient population decrease and eventual recovery when switched to feed containing 200 mg of a secondary substrate per liter.
摘要
在初始葡萄糖浓度与第二种底物（木糖）浓度相等的情况下，大肠杆菌的分批培养表现为底物的顺序利用，而在进料中两种底物浓度相等的连续培养系统中，无论稀释率高低，两种底物都会被完全利用。在低稀释率下处于稳态的此类系统，当突然转换到较高稀释率时，会经历种群密度的短暂下降，同时伴随着第二种底物的积累，但几乎没有葡萄糖的积累。研究发现，每升含200毫克葡萄糖的稳态系统，在转换为每升含200毫克第二种底物的进料时，会经历种群数量的短暂减少并最终恢复。
相似文献
1
Batch- and continuous-culture transients for two substrate systems.两种底物系统的分批培养和连续培养瞬变过程。
Appl Microbiol. 1972 Feb;23(2):354-9. doi: 10.1128/am.23.2.354-359.1972.
2
Cultivation of Escherichia coli with mixtures of 3-phenylpropionic acid and glucose: steady-state growth kinetics.用3-苯丙酸和葡萄糖混合物培养大肠杆菌：稳态生长动力学
Appl Environ Microbiol. 1997 Jul;63(7):2619-24. doi: 10.1128/aem.63.7.2619-2624.1997.
3
Efficient ethanol production from glucose, lactose, and xylose by recombinant Escherichia coli.重组大肠杆菌从葡萄糖、乳糖和木糖高效生产乙醇。
Appl Environ Microbiol. 1989 Aug;55(8):1943-8. doi: 10.1128/aem.55.8.1943-1948.1989.
4
Temperature-dependent growth kinetics of Escherichia coli ML 30 in glucose-limited continuous culture.葡萄糖限制连续培养中大肠杆菌ML 30的温度依赖性生长动力学
J Bacteriol. 1996 Aug;178(15):4530-9. doi: 10.1128/jb.178.15.4530-4539.1996.
5
Salt accumulation resulting from base added for pH control, and not ethanol, limits growth of Thermoanaerobacteriumthermosaccharolyticum HG-8 at elevated feed xylose concentrations in continuous culture.在连续培养中，为控制pH值而添加的碱（而非乙醇）导致的盐分积累，限制了嗜热栖热放线菌HG-8在高进料木糖浓度下的生长。
Biotechnol Prog. 2001 Jan-Feb;17(1):118-25. doi: 10.1021/bp000158n.
6
Growth kinetics of Escherichia coli with galactose and several other sugars in carbon-limited chemostat culture.在碳源受限的恒化器培养中，大肠杆菌利用半乳糖和其他几种糖类的生长动力学。
Can J Microbiol. 2000 Jan;46(1):72-80. doi: 10.1139/cjm-46-1-72.
7
Comparative stability of ethanol production by Escherichia coli KO11 in batch and chemostat culture.
J Ind Microbiol Biotechnol. 1999 Jul;23(1):701-8. doi: 10.1038/sj.jim.2900690.
8
Kinetics of the simultaneous utilization of sugar mixtures by Escherichia coli in continuous culture.大肠杆菌在连续培养中对糖混合物的同时利用动力学
Appl Environ Microbiol. 1996 May;62(5):1493-9. doi: 10.1128/aem.62.5.1493-1499.1996.
9
Dynamic flux balance modeling of microbial co-cultures for efficient batch fermentation of glucose and xylose mixtures.微生物共培养物的动态通量平衡建模，以实现葡萄糖和木糖混合物的高效批式发酵。
Biotechnol Bioeng. 2011 Feb;108(2):376-85. doi: 10.1002/bit.22954.
10
Is Escherichia coli growing in glucose-limited chemostat culture able to utilize other sugars without lag?在葡萄糖受限的恒化器培养中生长的大肠杆菌能否无延迟地利用其他糖类？
Microbiology (Reading). 1995 Jan;141 ( Pt 1):71-8. doi: 10.1099/00221287-141-1-71.
引用本文的文献
1
Enhancing CO-Valorization Using for Sustainable Fuel and Chemicals Production.利用……强化协同 valorization 以实现可持续燃料和化学品生产 。
注：“valorization”这个词可能有误，正确的可能是“valorization”，意思是“增值、 valorization” 等，这里暂且按原样翻译。
Front Bioeng Biotechnol. 2020 Mar 27;8:204. doi: 10.3389/fbioe.2020.00204. eCollection 2020.
2
Cauliflower waste incorporation into cane molasses improves ethanol production using Saccharomyces cerevisiae MTCC 178.向甘蔗蜜中添加菜花废料可提高使用酿酒酵母 MTCC 178 生产乙醇的产量。
Indian J Microbiol. 2007 Dec;47(4):353-7. doi: 10.1007/s12088-007-0063-1. Epub 2008 Jan 11.
3
Submerged conidiation and product formation by Aspergillus niger at low specific growth rates are affected in aerial developmental mutants.黑曲霉在低比生长速率下的淹没式产孢和产物形成受到气生发育突变体的影响。
Appl Environ Microbiol. 2011 Aug;77(15):5270-7. doi: 10.1128/AEM.00118-11. Epub 2011 Jun 7.
4
Transcriptomic insights into the physiology of Aspergillus niger approaching a specific growth rate of zero.转录组学揭示了接近特定生长速率为零的黑曲霉的生理特征。
Appl Environ Microbiol. 2010 Aug;76(16):5344-55. doi: 10.1128/AEM.00450-10. Epub 2010 Jun 18.
5
Transcriptomic comparison of Aspergillus niger growing on two different sugars reveals coordinated regulation of the secretory pathway.黑曲霉在两种不同糖类上生长的转录组学比较揭示了分泌途径的协同调控。
BMC Genomics. 2009 Jan 23;10:44. doi: 10.1186/1471-2164-10-44.
6
Dynamics of Substrate Consumption and Enzyme Synthesis in Chelatobacter heintzii during Growth in Carbon-Limited Continuous Culture with Different Mixtures of Glucose and Nitrilotriacetate.希瓦氏菌属在葡萄糖和氮川三乙酸不同混合碳限制连续培养中的生长过程中基质消耗和酶合成的动力学。
Appl Environ Microbiol. 1996 Jan;62(1):133-40. doi: 10.1128/aem.62.1.133-140.1996.
7
Xylose and Glucose Utilization by Bacteroides xylanolyticus X5-1 Cells Grown in Batch and Continuous Culture.木聚糖降解芽胞杆菌 X5-1 细胞在分批和连续培养中的木糖和葡萄糖利用。
Appl Environ Microbiol. 1994 Feb;60(2):576-80. doi: 10.1128/aem.60.2.576-580.1994.
8
Fermentation of xylose and rice straw hydrolysate to ethanol by Candida shehatae NCL-3501.嗜杀假丝酵母NCL-3501将木糖和稻草水解产物发酵为乙醇
J Ind Microbiol. 1996 Jul;17(1):20-3. doi: 10.1007/BF01570143.
9
Role of catabolite regulatory mechanisms in control of carbohydrate utilization by the rumen anaerobic fungus Neocallimastix frontalis.分解代谢调节机制在瘤胃厌氧真菌新美鞭菌属额叶菌碳水化合物利用控制中的作用。
Appl Environ Microbiol. 1983 Dec;46(6):1331-8. doi: 10.1128/aem.46.6.1331-1338.1983.
10
Sequential utilization of mixed monosaccharides by yeasts.酵母对混合单糖的顺序利用
Appl Environ Microbiol. 1982 Apr;43(4):840-5. doi: 10.1128/aem.43.4.840-845.1982.
本文引用的文献
1
Description of the chemostat.恒化器的描述。
Science. 1950 Dec 15;112(2920):715-6. doi: 10.1126/science.112.2920.715.
2
Determination of galactosamine and N-acetylgalactosamine in the presence of other hexosamines with galactose oxidase.在存在其他己糖胺的情况下，用半乳糖氧化酶测定半乳糖胺和N-乙酰半乳糖胺。
Anal Biochem. 1965 Sep;12(3):509-15. doi: 10.1016/0003-2697(65)90217-4.
3
Gravity flow system for continuous culture feed.用于连续培养进料的重力流系统。
Appl Microbiol. 1967 Jan;15(1):197. doi: 10.1128/am.15.1.197-.1967.
4
Measurement of unsteady state growth rates of micro-organisms.
Nature. 1965 Oct 16;208(5007):263-5. doi: 10.1038/208263a0.
5
Metabolic regulation in glucose-limited chemostat cultures of Escherichia coli.葡萄糖受限恒化器培养的大肠杆菌中的代谢调控
J Bacteriol. 1970 Nov;104(2):698-706. doi: 10.1128/jb.104.2.698-706.1970.
6
Automated counting of microbial colonies.微生物菌落的自动计数。
Appl Microbiol. 1970 Nov;20(5):815-20. doi: 10.1128/am.20.5.815-820.1970.
Zotero 插件