Ashton D H
Appl Microbiol. 1971 Jan;21(1):38-40. doi: 10.1128/am.21.1.38-40.1971.
Pea extract contains a factor which improves recovery counts of heat-stressed putrefactive anaerobe spores in a complex medium up to threefold. The factor is heat-stable and nondialyzable. Most of the active principle is found in the precipitate which forms during storage of pea extract at 4 C. The precipitate disperses upon heating, is high in starch content, and retains activity after extraction with organic solvents and water. Treatment of pea extract with alpha-amylase results in complete destruction of the active principle. These observations indicate that starch is the factor in pea extract responsible for increased recovery counts of heat-stressed putrefactive anaerobe spores.
豌豆提取物含有一种因子，在复合培养基中，它可使热应激腐败厌氧菌孢子的回收率提高至三倍。该因子耐热且不可透析。大部分活性成分存在于豌豆提取物在4℃储存时形成的沉淀物中。沉淀物加热后会分散，淀粉含量高，经有机溶剂和水提取后仍保留活性。用α-淀粉酶处理豌豆提取物会导致活性成分完全被破坏。这些观察结果表明，淀粉是豌豆提取物中导致热应激腐败厌氧菌孢子回收率增加的因子。
