Moats W A
J Bacteriol. 1971 Jan;105(1):165-71. doi: 10.1128/jb.105.1.165-171.1971.
Experimental observations on thermal injury and death of bacteria in the stationary phase can be explained by assuming that death results from inactivation of (X(L)) of N critical sites. It is assumed: (i) that inactivation of individual sites occurs at random and follows first-order kinetics, (ii) that the critical sites are identical and of equal heat resistance, and (iii) the bacterial population is homogeneous in heat resistance. A method is described for calculating k (the rate constant for inactivation of individual sites), N (sites per cell), and X(L) (the number which must be inactivated to cause death under the experimental conditions used) from experimental data. Theoretical curves calculated by using this model are identical with experimental curves, providing support for the assumptions used. Calculated values of N and X(L) were 130 and 21.7 for Pseudomonas viscosa and 175 and 2.7 for Salmonella anatum. There is considerable uncertainty in the absolute values of N, but they are probably > 100. It is predicted that X(L) will vary depending on the recovery medium used after heating. This theory is consistent with all experimental observations on thermal injury and death of bacteria.
关于稳定期细菌热损伤和死亡的实验观察结果，可以通过假设死亡是由 N 个关键位点的(X(L))失活导致来解释。假设如下：(i) 单个位点的失活是随机发生的，且遵循一级动力学；(ii) 关键位点是相同的，且具有相同的耐热性；(iii) 细菌群体在耐热性方面是均匀的。描述了一种根据实验数据计算 k（单个位点失活的速率常数）、N（每个细胞的位点数）和 X(L)（在所用实验条件下导致死亡必须失活的数量）的方法。使用该模型计算的理论曲线与实验曲线相同，为所用假设提供了支持。粘性假单胞菌的 N 和 X(L)计算值分别为 130 和 21.7，鸭沙门氏菌的计算值分别为 175 和 2.7。N 的绝对值存在相当大的不确定性，但它们可能大于 100。预计 X(L)会因加热后使用的复苏培养基而异。该理论与关于细菌热损伤和死亡的所有实验观察结果一致。
