Lehmann R, Hauss W H
Paroi Arterielle. 1979 Apr;5(1):17-22.
With a new method employing acridine organe (AO) as an ultracytochemical probe DNA template activity could be visualized within cell nuclei of the thoracic aortic wall of rabbits. After experimentally produced chronic renal hypertension nuclei with a relatively high number of AO chromatin interaction products were found in the endothelium, in the intimal region, in the outer third of the media, and in the adventiia indicating increased DNA template activity. This representative distribution pattern of DNA template activity was not found in comparable regions of the thoracic aorta of normotensive rabbits. The results are consistent with the current concept that cellular response to environmental stimuli occurs via activation of previously repressed genes.
采用吖啶橙（AO）作为超微细胞化学探针的新方法，可以观察到兔胸主动脉壁细胞核内的DNA模板活性。实验性诱发慢性肾性高血压后，在内皮、内膜区域、中膜外三分之一和外膜中发现了数量相对较多的AO染色质相互作用产物的细胞核，这表明DNA模板活性增加。在正常血压兔胸主动脉的类似区域未发现这种具有代表性的DNA模板活性分布模式。这些结果与当前的概念一致，即细胞对环境刺激的反应是通过激活先前被抑制的基因来实现的。
