Kerényi T, Lehmann R, Voss B, Jellinek H
Semmelweis OTE II. Kórbonctani Intézete, Bochum.
Morphol Igazsagugyi Orv Sz. 1990 Jan;30(1):28-35.
Interaction of monocytes and endothelial cells were examined by acridine orange reaction in arteries of normotensive and hypertonic rats. The method is suitable for electron microscope study of DNA template activity. Activity of gen was compared in surface bound and subendothelial and in nonadhesive endothelial cells bound to monocyte. Acridine orange positivity, indicative of genic activity, was not found in few monocytes adhered in arteries of normotensive animals, while 62% of adhesive monocytes of hypertonic and 86% of ones accessed to endothelial space contained the products of acridine-orange-chromatin reaction in its nuclear, showing with it the gene derepression quickly ensuing in acute hypertensive vascular lesions. Only 2% of endothelial cells of pseudo-operated normotensive animals showed acridine orange positivity, while 17% of endothelial cells of hypertonic animals were positive. In the latter animals, 57% of endothelial cells showed acridine orange positivity, if monocytes adhered to their surface. In 84% of monocyte-endothelial pairs connected to each other, the nuclei have activity of same sign. Results show intensive activation of genes of monocytes in relation with adhesion and migration to vascular wall. Presumably, both hypertonia and monocyte adhesion have contributed to the increase of template activity of endothelial cells.
通过吖啶橙反应检测正常血压大鼠和高血压大鼠动脉中单核细胞与内皮细胞的相互作用。该方法适用于DNA模板活性的电子显微镜研究。比较了单核细胞表面结合、内皮下结合以及与单核细胞结合的非粘附内皮细胞中的基因活性。在正常血压动物动脉中粘附的少数单核细胞中未发现吖啶橙阳性，这表明存在基因活性，而在高血压动物中，62%的粘附单核细胞和86%进入内皮间隙的单核细胞在其细胞核中含有吖啶橙染色质反应产物，这表明在急性高血压血管病变中基因去抑制迅速发生。假手术正常血压动物中只有2%的内皮细胞显示吖啶橙阳性，而高血压动物中有17%的内皮细胞呈阳性。在后者动物中，如果单核细胞粘附于其表面，57%的内皮细胞显示吖啶橙阳性。在84%相互连接的单核细胞-内皮细胞对中，细胞核具有相同的活性。结果表明，单核细胞基因与粘附和向血管壁迁移有关的强烈激活。推测，高血压和单核细胞粘附都有助于内皮细胞模板活性的增加。
