Tilney N L, Gowans J L
J Exp Med. 1971 May 1;133(5):951-62. doi: 10.1084/jem.133.5.951.
Pedicles of skin which lacked a lymphatic drainage were raised on the backs of rats in order to study the importance of afferent lymphatics in sensitization by skin allografts. Although allografts transplanted to the alymphatic pedicles enjoyed a prolonged survival, they contracted progressively from about 3 wk after transplantation and were reduced eventually to small scars. In contrast, autografts survived unchanged in size for the life-span of the pedicles which carried them. The slow contracture of the allografts was associated with sensitization of the host because test allografts applied orthotopically were destroyed with a second-set tempo. No regeneration of lymphatics from the long-standing pedicles could be demonstrated, and it was concluded that sensitization had occurred eventually through the blood, presumably by the process of peripheral sensitization. Allografts on skin pedicles could be destroyed rapidly by active or adoptive immunization, so it is probable that the level of sensitization to which they themselves gave rise was a low one. Although it is not disputed that afferent lymphatics are essential for the rapid destruction of skin allografts, it is clear that the absence of a lymphatic supply does not permanently exempt them from immunological attack in the rat.
为了研究传入淋巴管在皮肤同种异体移植致敏中的重要性，在大鼠背部掀起缺乏淋巴引流的皮肤蒂。尽管移植到无淋巴蒂上的同种异体移植有延长的存活期，但它们从移植后约3周开始逐渐收缩，最终缩小为小疤痕。相比之下，自体移植在携带它们的蒂的寿命期间大小保持不变。同种异体移植的缓慢挛缩与宿主的致敏有关，因为原位应用的试验同种异体移植以二次反应的速度被破坏。未证明长期存在的蒂有淋巴管再生，并且得出结论，致敏最终是通过血液发生的，大概是通过外周致敏过程。皮肤蒂上的同种异体移植可通过主动或过继免疫迅速破坏，因此它们自身引起的致敏水平可能较低。虽然传入淋巴管对于皮肤同种异体移植的快速破坏是必不可少的这一点并无争议，但很明显，缺乏淋巴供应并不会使它们在大鼠中永久免受免疫攻击。
