Miller H C, Cudkowicz G
J Exp Med. 1971 May 1;133(5):973-86. doi: 10.1084/jem.133.5.973.
Quantitative and qualitative changes of mouse bone marrow cells were studied by limiting dilution assays 2-3.5 months after immunization of donors with sheep erythrocytes or unrelated antigens (Salmonella typhimurium, horse and chicken erythrocytes). Irradiated (C3H x C57BL/10)F(1) mice were reconstituted with an excess of nonprimed thymocytes and small graded numbers of primed bone marrow cells. Direct and indirect plaque-forming cells (PFC) were induced by secondary stimulation with SRBC and enumerated on the 9th day after cell transplantation. Marrow precursors of PFC (P-PFC) cooperated with thymocytes in the production of direct and indirect PFC after SRBC priming. The limiting dilution plots, which were not compatible with predictions of the Poisson model before immunization, changed and conformed to this model afterwards, as if the population of P-PFC had become functionally more homogeneous. The concentration of marrow P-PFC increased up to the 3rd month after priming, and decreased during the 4th, varying over two logarithms of nucleated marrow cells. The fluctuation was simultaneous and of the same order of magnitude for precursors of direct and indirect PFC, which were class restricted. A third effect of immunization was detected at 3.5 months: individual precursor units generated 3-4 times more direct and indirect PFC than at earlier intervals. Qualitative and quantitative changes of marrow P-PFC participating in anti-sheep responses were specific, since antigens unrelated to SRBC failed to induce them. The data suggested that marrow-derived cells were the major carriers of immunologic memory, but that they functioned in cooperation with thymus-derived inducer cells during secondary anti-sheep responses.
在用绵羊红细胞或无关抗原（鼠伤寒沙门氏菌、马和鸡红细胞）免疫供体2至3.5个月后，通过有限稀释分析研究了小鼠骨髓细胞的定量和定性变化。用过量的未致敏胸腺细胞和少量分级的致敏骨髓细胞重建受辐照的（C3H×C57BL/10）F1小鼠。通过用SRBC进行二次刺激诱导直接和间接空斑形成细胞（PFC），并在细胞移植后第9天进行计数。PFC的骨髓前体细胞（P-PFC）在SRBC致敏后与胸腺细胞协同产生直接和间接PFC。免疫前与泊松模型预测不相符的有限稀释图，在免疫后发生了变化并符合该模型，就好像P-PFC群体在功能上变得更加同质。骨髓P-PFC的浓度在致敏后第3个月前升高，在第4个月期间下降，在有核骨髓细胞的两个对数范围内变化。直接和间接PFC的前体细胞的波动是同步的，且幅度相同，它们受类别限制。在3.5个月时检测到免疫的第三个效应：单个前体单位产生的直接和间接PFC比早期间隔时多3至4倍。参与抗绵羊反应的骨髓P-PFC的定性和定量变化是特异性的，因为与SRBC无关的抗原未能诱导这些变化。数据表明，骨髓来源的细胞是免疫记忆的主要载体，但在二次抗绵羊反应期间，它们与胸腺来源的诱导细胞协同发挥作用。
